Cell Culture Engineering VII

Tentative Oral Session Program Schedules

Oral Session #1. Virus and Vector Production in Cell Culture for Vaccines and Gene Therapy
Bernard Massie (Biotechnology Research Institute, Montreal) & John Aunins (Merck)
Sunday February 6 from 8:30-11:00 AM
8:30 - 8:34 AM Introduction; John G. Aunins, Merck Research Laboratories
8:34 - 8:58 AM PRODUCTION OF TISSUE SPECIFIC ADENOVIRUS VECTORS FOR GENE THERAPY
Thomas J. Wickham, GenVec, Inc.
8:58 - 9:03 AM Discussion
9:03 - 9:27 AM PROTEASE-DELETED ADENOVIRUS VECTORS AND COMPLEMENTING CELL LINES: POTENTIAL APPLICATIONS OF SINGLE-ROUND REPLICATION MUTANTS FOR VACCINATION AND GENE THERAPY
Bernard Massie; BRI/CNRC, University of Quebec, University of Montreal
9:27 - 9:32 AM Discussion
9:32 - 10:02 AM Coffee Break
10:02 - 10:26 AM CELL SURFACE BINDING AND INTRACELLULAR TRAFFICKING OF ADENOVIRAL GENE TRANSFER VECTORS IN DENDRITIC CELLS
Philip L. Leopold, Weill Medical College of Cornell University
10:26 - 10:31 AM Discussion
10:31 - 10:55 AM COMPARISON OF DIFFERENT BIOREACTOR SYSTEMS FOR THE PRODUCTION OF HIGH TITER RETROVIRAL VECTORS
Otto W. Merten, Genethon III
10:55 - 11:00 AM Discussion

Oral Session #2. Cell Physiology and Metabolism
Lena Haggstrom (KTH, Sweden) & Chris Hwang (Genzyme)
Sunday February 6 from 5:00-7:15 PM
5:00 – 5:05 PM Introduction; Chris Hwang, Genzyme
5:05 – 5:30 PM REGULATION OF CELL RESPONSES BY AUTOCRINE FACTORS
Douglas Lauffenburger, MIT
5:30 – 5:35 PM Discussion
5:35 – 5:55 PM METABOLIC DIVERSITY AND AUTOCRINE FACTORS IN SF9 INSECT CELLS
Lena Haggstrom, Royal Institute of Technology (Sweden)
5:55 – 6:00 PM Discussion
6:00 – 6:20 PM THE ROLE OF OF IGF IN THE GROWTH AND SURVIVAL OF CHO CELLS
Noelle-Anne Sunstrom, University of New South Wales
6:20 – 6:25 PM Discussion
6:25 – 6:45 PM THE ROLE OF METABOLISM IN THE APOPTOTIC CELL DEATH OF MAMMALIAN CELL CULTURES
R. Robert Balcarcel
Vanderbilt University, TN, USA
6:45 – 6:50 PM Discussion
6:50 – 7:10 PM METABOLIC CONTROL, GENE EXPRESSION ANALYSIS AND MATHEMATIC MODELING OF STEADY STATE MULTIPLICITY IN CELL CULTURE BIOREACTOR
Wei-Shou Hu, University of Minnesota
7:10 – 7:15 PM Discussion

Oral Session #3. Cell Production for Cell and Gene Therapies
Sean Forestell (SyStemix) and Bernhard Palsson (University of California, San Diego)
Monday February 7 from 8:30-10:00 AM
8:30 - 8:33 AM Introduction; Bernhard Palsson; University of California, San Diego
8:33 - 8:57 AM IMPLEMENTATION OF DENDRITIC CELL PROCESSING FOR CANCER IMMUNOTHERAPY
Madhusudan Peshwa, Dendreon
8:57 - 9:02 AM Discussion
9:02 - 9:26 AM IS THE SUCCESS OF CELL BASED IMMUNOTHERAPIES COMPROMISED BY THE CELL CULTURE PROTOCOLS BEING USED?
E. Terry Papoutsakis, Northwestern Univeristy
9:26 - 9:31 AM Discussion
9:31 - 9:55 AM DEVELOPMENT OF A SERUM-FREE PROCESS FOR THE EFFICIENT TRANSDUCTION OF HEMATOPOIETIC CELLS USED IN RETROVIRAL GENE THERAPY AGAINST HIV
Sean Forestell, SyStemix
9:55 - 10:00 AM Discussion

Oral Session #4. Protein Quality/Glycosylation
Nigel Jenkins (Lilly) & Manuel Carrondo (IBET, Portugal)
Monday February 7 from 10:00 AM - 12:15 PM
10:30 - 10:35 AM Introduction; Nigel Jenkins, Eli Lilly
10:35 - 11:00 AM CURIOUS DISCOVERIES OBSERVED IN THE QUEST FOR A WELL-CHARACTERIZED PROTEIN PHARMACEUTICAL
Fred Jacobson, Genentech
11:00 - 11:05 AM Discussion
11:05 - 11:30 AM RECENT ADVANCES IN TECHNIQUES FOR PRODUCT QUALITY ASSESSMENT OF BIOPHARMACEUTICALS
Ralph Riggin, Eli Lilly
11:30 - 11:35 AM Discussion
11:35 - 11:50 AM RECOMBINANT FURIN: TOOL TO ENSURE ENDOPROTEOLYTIC TARGET PROTEIN PRECURSOR PROCESSING
Uwe Schlokat, Baxter Healthcare
11:50 - 11:55 AM Discussion
11:55 AM - 12:10 PM INTRACELLULAR LOCALIZATION AND CHARACTERIZATION OF INCORRECTLY PROCESSED RECOMBINANT PROTEINS IN BACULOVIRUS-INFECTED INSECT CELLS
Volker Jaeger, GBF, Germany
12:10 - 12:15 PM Discussion
12:15 Concluding Remarks; Manuel Carrondo, IBET

Oral Session #5. Cell and Metabolic Engineering
Terry Papoutsakis (Northwestern University) & Lynne Krummen (Genentech)
Tuesday February 8 from 8:30-11:00 AM
8:30 - 8:34 AM Introduction; Lynne Krummen, Genentech
8:34 - 8:58 AM DEVELOPMENT OF NOVEL HUMAN-COMPATIBLE GENE REGULATION SYSTEMS WHICH ARE RESPONSIVE TO STREPTOGRAMIN ANTIBIOTICS
Martin Fussenegger, ETH Zurich
8:58 - 9:03 AM Discussion
9:03 - 9:27 AM ENGINEERING GLYCOSYLATION ENGINEERING IN INSECT CELLS
Michael Batenbaugh, John Hopkins University
9:27 - 9:32 AM Discussion
9:32 - 10:02 AM Coffee Break
10:02 - 10:26 AM IMPROVING GLYCOSYLATION OF RECOMBINANT GLYCOPROTEINS BY METABOLIC ENGINEERING OF UDP-GALACTOSE AND CMP-NANA POLLS IN CHO CELLS
Thomas Ryll, Genentech
10:26 - 10:31 AM Discussion
10:31 - 10:55 AM ENGINEERING MRNA TRANSLATION FOR INITIATION IN CHINESE HAMSTER OVARY CELLS FOR ENHANCED PRODUCTION OF RECOMBINANT PROTEINS
David C. James, University of Kent
10:55 - 11:00 AM Discussion

Oral Session #6. Analysis of Signal Transduction Pathways
Greg Stephanopoulos (MIT) & Michael Liebman (Roche Bioscience)
Tuesday February 8 from 6:30-7:30 PM
6:30 - 6:55 PM Introduction and SIGNAL TRANSDUCTION: THE NEW FRONTIER IN CHIP TECHNOLOGY AND NETWORK ANALYSIS
Gregory Stephanopoulos, MIT
6:55 - 7:00 PM Discussion
7:00 - 7:25 PM PATHWAY MODELING IN DRUG DEVELOPMENT: ANALYSIS OF SIGNAL TRANSDUCTION PATHWAYS AND CLOSING
Michael Liebman, Roche Bioscience
7:25 - 7:30 PM Discussion

Oral Session #7. High Throughput Gene Expression
Georg Schmid (Hoffman LaRoche) & Jamie Piret (University of British Columbia)
Wednesday February 9 from 8:30-10:00 AM
8:30 - 8:55 AM NON-VIRAL LARGE SCALE TRANSIENT EXPRESSION OF PROTEINS IN MAMMALIAN CELLS
Martin Jordan, Ecole Polytechnique Fédérale de Lausanne
8:55 - 9:00 AM Discussion
9:00 - 9:25 AM RAPID SELECTION OF HIGH-EXPRESSING CHO CLONES USING FLUORESCENCE-ACTIVATED CELL SORTING
Peter P. Gray, University of New South Wales
9:25 - 9:30 AM Discussion
9:30 - 9:55 AM DELPHI: A NEW TECHNIQUE TO DISCOVER BIOLOGICALLY RELEVANT PROTEINS
Martin F. Bachmann, Cytos Biotechnology
9:55 - 10:00 AM Discussion

Oral Session #8. Genomics and Proteomics
Mike Betenbaugh (Johns Hopkins University) & Paul Spence (Monsanto)
Wednesday February 9 from 10:30 AM - 12:15 PM
10:30 - 10:40 AM INTRODUCTION TO THE IMPACT OF GENOMICS ON CELL BIOLOGY
Paul Spence, Monsanto/Searle
10:40 - 11:05 AM THE ANALYSIS OF TRANSCRIPTIONAL PROFILING DATA
Dave Ficenec, Millenium Pharmaceuticals
11:05 - 11:10 AM Discussion
11:10 - 11:35 AM HUMAN ADIPOCYTES, ALL FAT IS NOT THE SAME
Michael Briggs, Searle Pharmaceuticals
11:35 - 11:40 AM Discussion
11:40 AM - 12:05 PM YEAST TWO-HYBRID TECHNOLOGIES AND THE IDENTIFICATION OF DRUG CANDIDATES
Kathie Young, Wyeth-Ayerst
12:05 - 12:10 PM Discussion
12:10 - 12:15 PM Concluding Remarks; Michael Betenbaugh, Johns Hopkins University

Oral Session #9. Manufacturing Issues
Greg MacMichael (Lilly) & Sadettin Ozturk (Bayer)
Wednesday February 9 from 5:00-7:15 PM
5:00 - 5:05 PM Introduction; Sadettin Ozturk, Bayer
5:05 - 5:25 PM FROM CHAOS TO CONTROL. 20 YEARS OF DEVELOPMENTS IN MANUFACTURE OF MAMMALIAN CELL DERIVED PRODUCTS
Yashwant M. Deo, Medarex Inc.
5:25 - 5:30 PM Discussion
5:30 - 5:50 PM DESIGN GUIDELINES FOR THE CHOICE OF SCALE-DOWN SYSTEMS FOR LATE STAGE PROCESS DEVELOPMENT
Divakar Ramakrishnan, Eli Lilly
5:50 - 5:55 PM Discussion
5:55 - 6:15 PM THE CHALLANGE OF CONTRACT MANUFACTURING IN THE BIOTECHNOLOGY INDUSTRY
David Sherwood, Lonza Biologics
6:15 - 6:20 PM Discussion
6:20 - 6:40 PM DEVELOPMENT OF A TECHNOLOGY PLATFORM FOR PRODUCTION OF HUMAN MONOCLONAL ANTIBODIES IN RECOMBINANT CHO CELL CULTURE
David Y. H. Chang, BASF Bioresearch 6:40 - 6:45 PM Discussion
6:45 - 7:05 PM BIOPROCESS VALIDATION FOR THE PRODUCTION OF A MONOCLONAL ANTIBODY IN FED-BATCH CULTURE OF A MURINE MYELOMA
Enda Moran, GlaxoWellcome
7:05 - 7:10 PM Discussion
7:10 - 7:15 PM Closing Remarks; Greg MacMicheals, Eli Lilly

Oral Session #10. Cultured Cells and Tissues for Transplantation and as Model Systems
Carole Heath (Immunex) & David Wolf (BioHybrid Technology)
Thursday February 10 from 8:00-10:00 AM
8:00 - 8:10 AM Introduction; Carole Heath, Immunex
8:10 - 8:30 AM DEVELOPMENT OF IN VITRO MODELS OF HEAD INJURY AND GLAUCOMA: EXAMINING THE EFFECT OF STRESS AND STRAIN ON CELLS OF THE NERVOUS SYSTEM
Theresa Good, Texas A&M
8:30 - 8:35 AM Discussion
8:35 - 8:55 AM HEPATOCYTE CULTURE ON PLANAR GAS-PERMEABLE SUPPORTS FOR USE IN A LIVER ASSIST DEVICE
Paul DiMilla, Organogenesis
8:55 - 9:00 AM Discussion
9:00 - 9:20 AM CELL THERAPY IN THE TREATMENT OF DIABETES
David Wolf, BioHybrid Technology
9:20 - 9:25 AM Discussion
9:25 - 9:45 AM CONTROLLING CHONDROGENESIS BY TISSUE ENGINEERING
Lawrence Bonassar, Center for Tissue Engineering, University of Massachusetts Medical School
9:45 - 9:50 AM Discussion
9:50 - 10:00 AM Concluding Remarks; David Wolf, BioHybrid Technology