Cell Culture Engineering VII Poster Sessions

Chairs
Frank Chaplen (Bioresource Engineering, Oregon State University)
Theresa Good (Chemical Engineering, Texas A&M University)
Sarah Harcum (Chemical Engineering, New Mexico State University)
Ron Taticek (Cell Culture/Fermentation R&D, Genentech, Inc.)

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Session I: Cell Host Engineering, Metabolism/Physiology & Protein Glycosylation

Protein Expression and Cell Cloning

1. ENGINEERING OF CELL HOST BY CELL FUSION TECHNIQUE AND ITS APPLICATION FOR PROTEIN PRODUCTION Myung-Sam Cho, Bayer Corp.

2. USE OF FLOW CYTOMETRY AND A MICROMANIPULATOR FOR HIGH-EFFICIENCY CLONING OF CELLS CO-EXPRESSING FLUORESCENT PROTEINS Bernard Massie, Biotechnology Research Institute, Montreal, Canada.

3. EXPRESSION OF PROTEINS IN CHO CELLS USING THE HOMOLOGOUS EF-1ALPHA GENE PROMOTER FROM CHINESE HAMSTER Daniel S Allison, ICOS Corporation

4. PROCESS DEVELOPMENT FOR THE PRODUCTION OF RECOMBINANT MONOCLONAL ANTIBODIES IN MYELOMA CELLS: COMPARISON OF DIFFERENT STRATEGIES FOR CELL LINE SELECTION. Adolfo Castillo Vitlloch, Center of Molecular Immunology

5. ENGINEERING CHO CELLS TO OVEREXPRESS A SECRETED GLYCOPROTEIN UPON INDUCTION Dhinakar S. Kompala, University of Colorado

6. HIGH-THROUGHPUT SCREENING AND SELECTION OF HIGH PRODUCERS BY A RAPID AND OBJECTIVE IMMOBILISED SURFACE DISPLAY TECHNIQUE Mohamed Al-Rubeai, University of Birmingham

7. HIGH THROUGHPUT RECOMBINANT PROTEIN PRODUCTION: TRANSIENT GENE EXPRESSION STRATEGIES Horst D. Blasey, Serono Pharmaceutical Research Institute

8. CELL ENCAPSULATION AND FLOW CYTOMETRY Hilary K. Metcalfe, Lonza Biologics PLC

9. AMPLIFIED GENES OF HIGHLY PRODUCIBLE CELL LINE LOCATED SPECIFIC CHROMOSOMAL REGION Tomohiro Yoshikawa, Dept. of Biotechnology, Graduate School of Engineering Osaka university

10. NOVEL TECHNIQUES AND APPLICATIONS AT THE INTERFACE OF CELL CULTURE AND GENOMICS/PROTEOMICS Georg Schmid, F. Hoffmann-La Roche Ltd.

11. USE OF FLOW CYTOMETRY TECHNIQUES AND THE ENHANCED GREEN FLUORESCENT PROTEIN IN THE DEVELOPMENT OF PRODUCTION CELL LINES, Kathleen Harris, Bayer Biotechnology

12. EFFECTIVE SELECTION METHOD FOR GENE AMPLIFIED CELL LINE USING FLOWCYTOMETER Tomohiro Yoshikawa, Dept. of Biotechnology, Graduate School of Engineering Osaka University

13. MANIPULATING THE STOICHIOMETRY BETWEEN STRUCTURAL PROTEINS FOR OPTIMUM PRODUCTION OF ROTAVIRUS-LIKE PARTICLES BY INSECT CELLS Octavio T. Ramirez, Instituto de Biotecnologia-UNAM

14. ASSESSMENT OF EPISOMAL VECTORS FOR THE GENERATION OF STABLE CELL LINES PRODUCING HIGH-LEVELS OF CHIMERIC ANTIBODIES Bernard Massie, Biotechnology Research Institute and Biomira, Inc., Canada

Cell Physiology, Metabolism & Metabolic Engineering

15. GROWTH FACTOR REQUIREMENTS FOR SERUM FREE CHO CELLS EXPRESSING TWO RECOMBINANT PROTEINS Arvia E. Morris, Immunex Corporation

16. QUANTITATIVE ANALYSIS OF METHYLGLYOXAL-DERIVED ADVANCED GLYCATION END PRODUCT (AGE) FORMATION Frank W.R. Chaplen, Oregon State University

17. MECHANISMS OF OSMOTIC STRESS RESPONSE IN HYBRIDOMA CELLS Susan Sharfstein, University of Toledo

18. OPTIMIZATION OF BHK CELL CULTURE BY METABOLISM MODULATION THROUGH NUTRIENT MANIPULATION Helder J. Cruz, IBET/ITQB

19. 293SF METABOLIC FLUX ANALYSIS DURING CELL GROWTH AND INFECTION WITH AN ADENOVIRAL VECTOR. I. Nadeau, Biotechnology Research Institute

20. APOPTOSIS, MITOCHONDRIA, AND POPULATION HETEROGENEITY IN MAMMALIAN CELL CULTURE Brian D. Follstad, Massachusetts Institute of Technology

21. ROLE OF INORGANIC SALTS AND VITAMINS IN DETERMINING APOPTOSIS AND EXTENT OF SUPPRESSION BY BCL-2 Mohamed Al-Rubeai, University of Birmingham

22. APPROACHES TO REDUCE APOPTOSIS IN CELL CULTURE PROCESSES Gary Pettman, R&D SmithKline Beecham Pharmaceuticals

23. USING METABOLIC FLUX ANALYSIS TO RESOLVE THE EFFECTS OF COMPARTMENTALIZATION ON GLUCO-GLUTAMINOLYSIS Kashif Sheikh, The University of Queensland

24. BIOPROCESS APPLICATIONS OF PCYTTS - COLD INDUCIBLE GENE EXPRESSION SYSTEM Marco Boorsma, Cytos Biotechnology AG

25. EXPRESSION OF HETEROLOGOUS PROTEINS BY QUIESCENT CHO CELLS OF HIGH VIABILITY Stefan Wildt, Department of Chemical Engineering, Massachusetts Institute of Technology

26. CULTURE pH AND PO2 ARE POTENT DETERMINANTS OF GRANULOCYTE PROLIFERATION AND DIFFERENTIATION: EXPERIMENTAL EVIDENCE AND MODEL VERIFICATION Diane L. Hevehan, Northwestern University

27. TOPOLOGICALLY SIGNIFICANT EFFECTS OF OXYGEN TENSION ON MEGAKARYOCYTIC DIFFERENTIATION AND MATURATION Sigma S. Mostafa, Northwestern University

28. EFFECTS OF ANTI-APOPTOSIS PROTEINS ON CELL BEHAVIOR IN MAMMALIAN CELL CULTURE Tina M. Sauerwald, Johns Hopkins University

29. MECHANISTIC STUDIES OF INTERFACIAL PHENOMENA IN CHO CELL CULTURES Chihae Yang, Chemistry Department, Otterbein College

30. INHIBITION OF APOPTOSIS IN BIOREACTORS BY ANTI-APOPTOTIC GENES Jana van de Goor, Genentech, Inc.

31. IN VIVO TITRATION OF THE ANTI-APOPTOTIC PROTEIN BCL-2 AND ITS EFFECT ON MITOCHONDRIAL MEMBRANE POTENTIAL OF CHO CELLS Stefan Wildt, Department of Chemical Engineering, Massachusetts Institute of Technology

32. EXPRESSION AND AMPLIFICATION OF GLUTAMINE SYNTHETASE GENE FOR CONSTRUCTING AMMONIA-METABOLIZING CELL LINES IN HYBRID BIOARTIFICIAL LIVER SUPPORT SYSTEM Takeshi Omasa, Department of Biotechnology, Graduate School of Engineering, Osaka University

33. OSMOPROTECTIVE EFFECT OF GLYCINE BETAINE ON FOREIGN PROTEIN PRODUCTION IN HYPEROSMOTIC RCHO CELL CULTURES Gyun Min Lee, KAIST

34. LACTIC ACID INHIBITION REVISITED THROUGH HEMATOPOIETIC CELL CULTURES Sanjay D. Patel, Northwestern University.

35. DEVELOPMENT OF NEW SYNTHETIC COPOLYMERS FOR CELL IMMUNOPROTECTION THERAPY J. Lukas, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic

36. GENERATION OF GABA-SYNTHESIZING NERVE CELLS CULTURED FROM EMBRYONIC CORTEX CEREBRI WITH AND WITHOUT CELL-TO-CELL CONTACTS A.S. Uppal, Department of Neurosciences, Government Medical College

37. PRODUCTION OF RECOMBINANT HUMAN BMP AND OSTEONECTIN WITH PROTEIN-FREE GROWING CHO CELLS AND IN VIVO OSTEOINDUCTION F. Messi, Cell Culture Technologies, Zurich, Switzerland.

38. AMMONIA REMOVAL USING HEPATOMA CELLS IN MAMMALIAN CELL CULTURE Ik-Hwan Kim, Graduate School of Biotechnology, Korea University

39. CELL MIGRATION THROUGH BIOMIMETIC HYDROGELS Andrea Gobin, Dept. of Bioengineering, Rice University, Houston, TX.

40. THE USE OF FLUORESCENT TIME LAPSE MICROSCOPY TO STUDY REPLICATION HISTORY OF ASYMMETRICALLY DIVIDING CELLS USING PKH26 Stephen S. Fong, Dept. of Bioengineering, UCSD, LaJolla, CA.

41. IMAGING THE RESPONSE OF GFP-ACTIN FILAMENTS AT THE FOCAL CONTACTS TO APPLIED FORCE OVER THE APICAL SURFACE OF ANCHORED HUVEC Anshu B. Mathur, Dept. of Biomedical Engineering, Duke University, Durham, NC.

42. NEW SYNTHETIC COPOLYMER DESIGNED FOR ENCAPSULATION OF DOPAMIN-PRODUCING CELLS: BIOCOMPATIBILITY AND CYTOTOXICITY TESTS J. Mokry, Department of Histology and Embryology, Medical Faculty, Charles University

Protein quality/glycosylation

43. EFFECT OF PROTEIN DISULPHIDE ISOMERASE OVEREXPRESSION ON RECOMBINANT PROTEIN SECRETION IN CHINESE HAMSTER OVARY CELLS Pranhitha Reddy, IMMUNEX, Seattle, WA

44. OSMOLALITY PLAYS A PREDOMINANT ROLE IN ALTERING MONOCLONAL ANTIBODY GLYCOSYLATION IN HYBRIDOMA CELLS UNDER HYPERCAPNIA Albert E. Schmelzer, Northwestern University

45. ENGINEERING SIALYLATION INTO INSECT CELLS: A BIOINFORMATICS APPROACH Shawn M. Lawrence, Johns Hopkins University

46. ANALYSIS OF THE OLIGOSACCHARIDE STRUCTURE OF HUMAN ERYTHROPOIETIN IN NORMAL AND AMMONIA-LOADED CULTURES Michael Butler, University of Manitoba

47. CONTROLLING N-GLYCOSYLATION SITE-OCCUPANCY OF A RECOMBINANT GLYCOPROTEIN EXPRESSED IN CHO CELLS Martin Gawlitzek, Genentech, Inc.

48. METABOLIC CONTROL OF RECOMBINANT PROTEIN N-GLYCOSYLATION IN NS0 AND CHO CELLS. Kym Baker, University of Kent

49. METABOLIC ENGINEERING OF RECOMBINANT PLASMINOGEN SIALYLATION IN CHO CELLS Kym Baker, University of Kent

50. CONTROL OF GLYCOSYLATION BY ENVIRONMENTAL MANIPULATIONS IN CHO CELL CULTURES Robert Kiss, Genentech, Inc.

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Session II: Large Scale Cell Culture

Bioreactor Engineering, Modeling, and Scale-Up

51. A PROPOSED METHODOLOGY FOR SCALE-UP AND SCALE-DOWN Jeffrey J. Chalmers, Ohio State University

52. HYBRIDOMA CELL RESPONSE MECHANISMS TO ELEVATED CARBON DIOXIDE AND HYPEROSMOTIC CULTURE CONDITIONS: AMINO ACIDS AS PROTECTIVE AGENTS Vivian M. de Zengotita, Department of Chemical Engineering, Northwestern University

53. SOLUTION TO THE HIGH DISSOLVED CO2 PROBLEM IN HIGH-DENSITY PERFUSION CULTURE OF MAMMALIAN CELLS R. Matanguihan, Bayer Corporation

54. A NOVEL TOOL FOR FERMENTER MONITORING AND CONTROL USING A MATHEMATICAL ANALYSIS OF ACID/BASE EQUILIBRIA IN CELL CULTURE Sadettin S. Ozturk, Bayer Corporation

55. LARGE-SCALE PREDICTION OF GENE EXPRESSION AND PROTEOMIC SHIFTS IN CONTINUOUS HYBRIDOMA CULTURES EXHIBITING STEADY STATE MULTIPLICITY Jeffrey D. Varner, Chemical Engineering and Materials Science, University of Minnesota

56. METABOLIC CONTROL ANALYSIS OF MONOCLONAL ANTIBODY SYNTHESIS R. González, Center for Biochemical Engineering and Biotechnology, University of Chile

57. SCALE-UP AND OPTIMIZATION OF HYBRIDOMA CELL GROWTH, IgG PRODUCTION AND DOWNSTREAM PROCESSING IN SERUM-FREE CHEMICALLY DEFINED MEDIA Glenn P. Godwin, Life Technologies, Inc.

58. QUANTIFICATION OF THE MAGNITUDE AND DISTRIBUTION OF THE LOCAL ENERGY DISSIPATION IN BIOREACTORS Ningning Ma, Ohio State University

59. PROCESS DEVELOPMENT FOR THE PRODUCTION OF RECOMBINANT MONOCLONAL ANTIBODIES IN MYELOMA CELLS: PILOT SCALE OPTIMIZATION OF HOLLOW FIBER BIOREACTOR CELL CULTURE AND PURIFICATION. Adolfo Castillo Vitlloch, Center of Molecular Immunology

60. SCALE-UP OF INSECT CELL CULTURES, A FOCUS ON THE INOCULATION DENSITY R.C.L. Marteijn, Wageningen University, Food and Bioprocess Engineering Group

Monitoring and Control of Cell Culture Processes

61. CONTROL AND OPTIMIZATION OF CHO CELL PERFUSION AND FED-BATCH CULTURES PRODUCING AN INHIBITORY RECOMBINANT PROTEIN Jason E. Dowd, Biotechnology Laboratory and Department of Chemical and Biological Engineering, University of British Columbia

62. ALTERNATIVE METHODS FOR BACULOVIRUS TITRATION R.V. Venkat , SmithKline Beecham Pharmaceuticals, King-of-Prussia, Pennsylvania.

63. CELL SIZE AS A TOOL TO PREDICT PROTEIN PRODUCTIVITY OF THE INSECT CELL-BACULOVIRUS EXPRESSION SYSTEM Laura Palomares, Instituto de Biotecnologia-UNAM

64. KINETICS OF BACULOVIRUS INFECTION, REPLICATION AND RELEASE USING REAL TIME PCR Lars Keld Nielsen, Dept. of Chemical Engineering, The University of Queensland, Australia.

65. EFFECT OF CONTROLLED CULTURE REDOX POTENTIAL ON APOPTOSIS AND HYBRIDOMA CELL CULTURE PARAMETERS Angelica Meneses, Instituto de Biotecnologia-UNAM

66. PROCESS CONTROL AND FEEDING STRATEGIES TO INCREASE YIELDS FROM CHINESE HAMSTER OVARY (CHO) CELLS IN HIGH DENSITY CELL CULTURES. Joseph Camire, HyClone Laboratories, Inc.

67. THE MEASUREMENT OF DISSOLVED CARBON DIOXIDE IN A MAMMALIAN MICROCARRIER CELL CULTURE BY USING AN IN SITU FIBER-OPTIC CHEMICAL SENSOR Robert N. Pattison, Genzyme Corporation, Bioengineering Group, Technology Development

68. A NEW METHOD TO MEASURE CELL DENSITY AND KINETICS OF CELL ATTACHMENT ON MICROCARRIERS Pradyumna Namdev, Schering-Plough Research Institute

69. 66. BIOMASS AND AGGREGATION ANALYSIS OF MAMMALIAN SUSPENSION CELLS BY PARTICLE SIZE MEASUREMENT Zhong Liu, Schering-Plough Research Institute

70. FLOW CYTOMETRIC ASSAYS FOR MONITORING CELLULAR RESPONSES OF CHO CELLS TO CHANGES IN CULTURE ENVIRONMENT Cynthia Hoy, Genentech, Inc.

71. ONLINE MONITORING OF PHYSIOLOGICAL PARAMETERS OF INSECT CELL CULTURES DURING THE GROWTH AND INFECTION PROCESS. Cynthia Elias, Biotechnology Research Institute (NRC)

72. IN SITU DETERMINATION OF PROTEIN TURN-OVER RATES OF CHO CELLS BY 13C-NMR SPECTROSCOPY. Hendrik Bonarius, Novo Nordisk, Gentofte, Denmark.

73. DESIGN, VALIDATION AND USE OF AN ON-LINE FLUOROMETER FOR THE MONITORING OF RECOMBINANT ADENOVIRUS PRODUCTION. P.-A. Gilbert, University of Laval and Biotechnology Research Institute, Quebec, Canada

74. EFFECTS OF CELL SPECIFIC PERFUSION RATE (CSPR) ON THE CELL CULTURE KINETICS IN A PERFUSION BIOREACTOR Sadettin S. Ozturk, Bayer Corporation

75. MONITORING ADENOVIRUS INFECTIONS WITH ON-LINE AND OFF-LINE METHODS T. Monica, Berlex Biosciences

Manufacturing Issues

76. DEVELOPMENT OF A HIERARCHICAL FRAMEWORK FOR MODELLING BIOPHARMACEUTICALMANUFACTURE SO AS TO ACHIEVE MORE EFFECTIVE PRODUCTION PLANNING Suzanne Farid, University College London

77. DEVELOPMENT AND OPTIMIZATION OF A DEFINED, SERUM-FREE MEDIUM AND FEED SUPPLEMENT SPECIFICALLY FORMULATED FOR THE HEK 293 CELL LINE Shelly Wilcox, JRH Biosciences

78. PILOT SCALE PRODUCTION OF HIV-1 gp120 IN PACKED-BED BIOREACTOR USING THE VACCINIA VIRUS /T7 EXPRESSION SYSTEM Joseph Shiloach, Biotechnology Unit, NIDDK, NIH, Bethesda, MD

79. ROBOT AUTOMATION OF SAMPLING AND SAMPLE MANAGEMENT DURING CULTIVATION OF MAMMALIAN CELLS IN PILOT SCALE Dirk Luetkemeyer, Institute of Cell Culture Technology

80. PLANT PROTEIN HYDROLYZATES: PREPARATION OF DEFINED PEPTIDE FRACTIONS SUPPORTING GROWTH AND SURVIVAL OF ANIMAL CELLS Frantisek Franek, Institute of Experimental Botany, Czech Academy of Sciences

81. DEVELOPMENT AND OPTIMIZATION OF PROTEIN-FREE CELL BANKING TECHNOLOGY John Thrift, Bayer Corporation

82. ONLINE CONTROLLED PERFUSED FLUIDIZED BED AND FED BATCH PRODUCTION ñ FEEDING STRATEGIES DESIGNED FOR THE HIGH QUALITY & HIGH PRODUCTIVITY MANUFACTURE OF RECOMBINANT HUMAN CLINICAL GRADE PROTEINS Dethardt Müller, Institute of Applied Microbiology

83. DEVELOPMENT OF A PRODUCTION SCALE PROCESS FOR RECOMBINANT THYROID STIMULATING HORMONE RECEPTOR (TSH-R) Stiens, L.R., InVivo GmbH

84. UPSTREAM PROCESS DEVELOPMENT FOR AN ANTIBODY FUSION PROTEIN Leo A. van der Pol, DSM-Biologics

85. OPTIMIZATION OF HYDROLYSATE MIXTURE CONCENTRATIONS IN SERUM-FREE MEDIA USING RESPONSE SURFACE EXPERIMENTAL DESIGNS Gregg Nyberg, Genetics Institute

86. GROWTH OF NS0 CELLS IN PROTEIN-FREE, CHEMICALLY DEFINED MEDIUM Stephen Gorfien, Life Technologies, Inc.

Cell Culture for Vaccines, Cell and Gene Therapies

87. PARAMETERS EFFECTING RETROVIRUS PRODUCTION DURING CULTURE OF THE FLYRD18 HUMAN PACKAGING CELL LINE Mohamed Al-Rubeai, University of Birmingham

88. APPLICATIONS OF A SERUM-FREE MEDIUM DEVELOPED FOR THE GROWTH OF VERO CELLS Michael Butler, University of Manitoba, Winnipeg, Canada

89. DEVELOPMENT OF CELL CULTURE CONDITIONS FOR LARGE SCALE RETROVIRAL VECTOR PRODUCTION IN REDUCED SERUM MEDIA Manish Singh, Chiron Corporation, Center for Gene Therapy

90. MDCK SINGLE CELL SUSPENSION CULTURE AS A NEW TOOL FOR INFLUENZA VACCINE PRODUCTION: CELL GROWTH AND VIRUS PRODUCTION IN CHEMICALLY DEFINED MEDIA Holger Lübben, Preclinical Development, Chiron Behring GmbH and Co

91. INTEGRATED OPTIMIZATION OF THE PRODUCTION OF RETROVIRUS FOR GENE THERAPY Pedro E. Cruz, IBET

92. PRODUCTION ADENOVIRUS GENE THERAPY VECTORS Crystal Auger, Genzyme Corporation

93. CHALLENGES WITH EX VIVO EXPANSION OF TUMOR- AND VIRAL-ANTIGEN SPECIFIC CYTOTOXIC T LYMPHOCYTES (CTL) FOR ADOPTIVE IMMUNOTHERAPY Madhusudan V. Peshwa, Dendreon Corporation

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