PROGRAM

ENZYME ENGINEERING CONFERENCE XVI


October 7 - 12, 2001
Seminaris See Hotel Potsdam
An der Pirschheide 40
D-14471 Potsdam, GERMANY
Tel: 49-(0)331-9090-7000 Fax: 49-(0)331-9090-705
www.seminaris.de

Conference Co-Chairs:
Professor Frieder W. Scheller
Institute of Biochemistry & Biology, University of Potsdam, Germany
Professor Christian Wandrey
Institute of Biotechnology, Research Centre Jülich, Germany
Professor Oreste Ghisalba
Novartis Pharma AG, Basel, Switzerland

UNITED ENGINEERING FOUNDATION, INC.
Three Park Avenue, 27th Floor
New York, NY 10016-5902
T: 1-212-591-7836 - F: 1-212-591-7441
E: engfnd@aol.com - www.engfnd.org/1ak.html

The conference co-chairs would like to thank the following sponsors:

Europe
Amino GmbH, Frellstedt, Germany
Aventis Crop Science, Frankfurt (AgrEvo), Germany
Aventis Pharma Deutschland GmbH, Frankfurt, Germany
BASF AG, Ludwigshafen, Germany
Bayer AG, Leverkusen, Germany
Bundesministerium für Bildung und Forschung, Bonn, Germany
CARPUS + PARTNER GmbH, Aachen, Germany
Ciba Specialty Chemicals AG, Basel, Switzerland
Consortium für elektrochemische Industrie, München, Germany
DASGIP mbH, Jülich, Germany
Degussa-Hüls AG, Standort Wolfgang, Germany
degussa, Düsseldorf, Germany
Deutsche Bundesstiftung Umwelt, Osnabrück, Germany
Dow Deutschland GmbH, Schwalbach, Germany
DSM Research, Geleen, The Netherlands
Hoffmann La-Roche AG, Basel, Switzerland
Fonds der Chemischen Industrie, Frankfurt, Germany
Forschungszentrum Jülich GmbH, Jülich, Germany
Georg Thieme Verlag, Stuttgart, Germany
Girindus AG, Bensberg, Germany
Grünenthal GmbH, Aachen, Germany
Henkel KGaA, Düsseldorf, Germany
Lonza AG, Visp, Switzerland
McKinsey&Company, München, Germany
Merck KGaA, Darmstadt, Germany
Novartis International AG, Basel, Switzerland
Novozymes, Bagsvaerd, Denmark
OECD, Organisation for Economic Co-Operation and Development, Paris, France
PricewaterhouseCoopers, Basel, Switzerland
RheinBiotech GmbH, Düsseldorf, Germany
Roche Diagnostics GmbH, Penzberg, Germany
Röhm GmbH, Darmstadt, Germany
ScheBo Tech GmbH, Giessen, Germany
Schering AG, Berlin, Germany
Serono S.A., Geneva, Switzerland
SOLVAY Pharmaceuticals GmbH, Hannover, Germany
SÜD-CHEMIE AG, Bruckmühl b. München, Germany
Verein der Freunde und Förderer der KFA, Jülich, Germany
Wiley-VCH Verlag GmbH, Weinheim

Japan
Ajinomoto Co., Inc., Kawasaki
Amano Enzymes Inc., Nagoya
Fuji Chemical Industries, Toyama
Japan Bioindustry Association (JBA), Tokyo
Japanese Society for Enzyme Engineering, Osaka
Kaneka Corporation, Osaka
Kyoto University, Applied Microbiology Institute, Kyoto
Novozymes Japan Ltd., Tokyo
Suntory Ltd., Osaka

USA
Genencor International, Inc., Palo Alto, CA
Merck & Co, Inc., Rahway, New Jersey


Sunday, October 7, 2001

15:00 - 18:00 Registration (Hotel Lobby)

16:00 - 18:00 Linking the Pharmaceutical Industry with Academia
Pre-conference Workshop by PricewaterhouseCoopers, Basel, Switzerland
Workshop Co-Chairs:
Andreas Fischer and Hugh Browne

18:00 - 19:30 Opening Dinner

19:30 - 19:45 Opening Remarks
Conference Co-Chair: Frieder W. Scheller, University of Potsdam, GERMANY
UEF Liaison: Allen Laskin, New York, USA

19:45 - 20:45 Plenary Lecture
Structures and Mechanisms of the Complexes from the Respiratory Chain
Hartmut Michel (Nobel Prize Laureate), Frankfurt, GERMANY

21:00 - 22.00 Opening Reception

NOTES

You must sign up for both the Monday excursion to Potsdam/University of Potsdam and the Wednesday excursion to Berlin. Sign-up sheets are at the conference secretariat office.

All technical sessions will be in the Seminar Rooms (Lobby Level).

All poster boards will be in the Lobby area.

All meals will be buffet style in the main Restaurant (Lobby Level).

All coffee breaks and social hours will be in the Lobby Area by the posters.

Speakers - keep to your allotted time and be sure to leave sufficient time for discussion.

No smoking please at any conference function.

Please turn off to turn your cellular telephone to vibrate during technical sessions.

Check the television monitor in your room for your messages. You can leave messages for other conference participants either at the hotel reception (for placement on the recipiant's bedroom television monitor) or on the message board at the conference secretariat office in the lobby area.

Vegetarians: There will be vegetarian selections at all buffets. However, please sign up at the conference secretariat office for a vegetarian boxed lunch for Wednesday's trip to Berlin.

Monday, October 8, 2001

06:30 - 08:15 Breakfast

08:30 - 12:00 SESSION I: INDUSTRIAL BIOCATALYSIS
Session Co-Chairs:
Christian Wandrey, Jülich Research Centre, Jülich, GERMANY
Poul B. Poulsen, NOVOZYMES, Bagsvaerd, DENMARK

08:30 - 09:00 Industrial Biocatalysis - The American View from Academia
Harvey W. Blanch, University of California-Berkeley, USA
Chi Huey Wong, The Scripps Research Institute, La Jolla, USA

09:00 - 09:30 Industrial Biocatalysis - The American View from Industry
Keith A. Powell, Maxygen Inc., Redwood City, USA

09:30 - 10:00 Industrial Biocatalysis - The Asian View from Academia
Sakayu Shimizu, Kyoto University, Kyoto, JAPAN

10:00 - 10:30 Coffee Break

10:30 - 11:00 Industrial Biocatalysis - The Asian View from Industry
Junzo Hasegawa, KANEKA Corp, Osaka, JAPAN

11:00 - 11:30 Industrial Biocatalysis - The European View from Academia
Herfried Griengl, Graz Technical University, Graz, AUSTRIA

11:30 - 12:00 Industrial Biocatalysis - The European View from Industry
Marcel Wubbolts, DSM Research Geleen, Geleen, THE NETHERLANDS

12:00 - 13:00 Lunch

13:30 - 17:30 (Buses leave at 13:30 promptly from front of hotel)
Visit to Potsdam/ad hoc Session at Potsdam University
(including a short reception at the University)
Session Chair:
Oreste Ghisalba, Novartis Pharma AG, Basel, SWITZERLAND

Biotechnology - An Enabling Technology for Industrial Sustainability
Alan T. Bull, University of Kent, UNITED KINGDOM

17:30 - 19:30 POSTER SESSION I
Session Chair:
Harvey W. Blanch, University of California, Berkeley, USA
(Presenters will be present to answer questions)

19:30 - 21:00 Dinner

21:00 - 22:30 Social hour (in poster area)

Tuesday, October 9, 2001

06:30 - 08:15 Breakfast

08:30 - 12:00 SESSION II: ANALYTICAL APPLICATIONS
Session Co-Chairs:
Gao Xiang Li, Institute of Microbiology, Chinese Academy of Sciences, Beijing, CHINA
Reinhard Renneberg, Hong Kong University of Science and Technology, Hong Kong, CHINA

08:30 - 09:00 Imaging Cytochrome P450: The Scanning Tunnelling Microscopy
of Redox Active Proteins
H. Allen O. Hill, Oxford, UNITED KINGDOM

09:00 - 09:30 Single Molecule Enzymology
Rudolf Rigler, Karolinska Institute, Stockholm, SWEDEN

09:30 - 10:00 Molecular Enzyme Engineering for Biosensors and Biochips
Xian-En Zhang, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, CHINA

10:00 - 10:30 Coffee Break

10:30 - 11:00 The Development of an Interface Protein to Facilitate the
Electron Transfer Between Sensor Enzyme and Electro-Chemical Reaction - The Artificial Electron Transfer Subunit
Koji Sode, Tokyo University of Agriculture and Technology, Tokyo, JAPAN

11:00 - 11:30 Biosensors for Creatinin and HbAl-c - Can Antibodies Make Them Better?
Axel Warsinke, University of Potsdam, GERMANY

11:30 - 12:00 Discussion

12:00 - 13:30 Lunch

14:00 - 17:00 SESSION III: NEW BIOCATALYSTS
Session Co-Chairs:
Yasuhisa Asano, Toyama Prefectural University, JAPAN
Johannes Paul Pachlatko, Syngenta AG, Basel, SWITZERLAND

14:00 - 14:30 Human Cytochrome P450 in Bacteria as a Tool for Drug Research and
Development
Thomas Friedberg, University of Dundee, UNITED KINGDOM

14:30 - 15:00 Exploring the Substrate Spectrum of Recombinant Sucrose
Synthase for Carbohydrate Engineering
Lothar Elling, Heinrich Heine University of Düsseldorf, GERMANY

15:00 - 15:30 Enzymatic Synthesis of Non-Protein Amino Acids Using
Cystein Synthetase Coupled with Acetyl-CoA Regeneration
Kazuhiro Nakanishi, Okayama University, JAPAN

15:30 - 16:00 Coffee Break

16:00 - 16:30 Synthesis of Cephalexin from D-2-Amino-2-Phenylacetonitrile
Using an Enzymatic Cascade
Fred van Rantwijk, Delft University of Technology, THE NETHERLANDS

16:30 - 17:00 Directed Evolution of Hydroxynitrillyase towards Improved
Activity with Bulky Substrates
Helmut Schwab, Technical University of Graz, AUSTRIA

17:30 - 19:30 POSTER SESSION II
Session Chair:
Hak-Sung Kim, Korea Advanced Institute of Science and Technology, Taejon, KOREA
(Poster presenters will be present to answer questions)

19:30 - 21:00 Dinner

21:00 - 22:30 Social hour (in poster area)

Wednesday, October 10, 2001

06:30 - 08:00 Breakfast

08:00 - 11:00 SESSION IV: DIRECTED EVOLUTION OF BIOCATALYSTS
Session Chair:
Karl-Erich Jaeger, Ruhr University of Bochum, GERMANY

08:00 - 08:30 The Limits of Thermotolerance
Andrew Ellington, University of Texas, Austin, USA

08:30 - 09:00 Molecular Breeding of Genes, Pathways and Genomes
by DNA Shuffling
Willem P.C. Stemmer, Maxygen, Inc., Redwood City, USA

09:00 - 09:30 Engineering of Lipase Activities
Sanne O. Schrøder Glad, Novozymes A/S, Smørmosevej, DENMARK

09:30 - 10:00 Coffee Break

10:00 - 10:30 Molecular Breeding of Biosynthetic Pathways
Claudia Schmidt-Dannert, University of Minnesota, St. Paul, USA

10:30 - 11:00 Creation of Enantioselective Lipases by Directed Evolution
Karl-Erich Jaeger, Ruhr University of Bochum, GERMANY

11:15 - 18:00 Tour to Berlin - Meet in hotel lobby by 11:15 as buses must leave by
11:30 sharp to keep first reservation. Be sure to bring your ticket
(in your name badge holder).
Lunch boxes will be distributed on the bus.

19:00 - 20:30 Dinner

20:30 - 22:30 Social hour (in poster area)

Thursday, October 11, 2001

06:30 - 08:15 Breakfast

08:30 - 12:00 SESSION V: STRUCTURE/CATALYTIC MECHANISMS
Session Co-Chairs:
Jean-Pierre Toutant, OECD Coordinator, Montpellier, FRANCE
Joel L. Sussman, The Weizmann Institute of Science, Rehovot, ISRAEL

08:30 - 09:00 Acetylcholinesterase: Is It Possible to Infer Alternate Functions for this
Enzyme from Its 3D Structure?
Joel I. Sussman, The Weizmann Institute of Science, Rehovot, ISRAEL

09:00 - 09:30 Substrate Recognition Scheme of Glycosyltrehalose Trehalohydrolase from
the Hyperthermophilic Archaeum Sulfolobus solfataricus
Michael D. Feese, Emerald BioStructures, Inc., USA

09:30 - 10:00 Cyclic Amidases, New 3D-Structures of Enzymes with Biotechnological
Interest
Dietmar Schomburg, University of Cologne, GERMANY

10:00 - 10:30 Coffee Break

10:30 - 11:00 Structures and Mechanisms of Chitinases
M.G. Peter, University of Potsdam, GERMANY

11:00 - 11:30 Characterization, Sequencing and Protein Engineering of Cryophilic
Proteases of Marine Origin
J.A. Asenjo, University of Chile, Santiago, CHILE

11:30 - 12:00 Discussion

12:00 - 13:00 Lunch

13:30 - 16.30 SESSION VI: WORKSHOP NEW TECHNIQUES
Session Co-Chairs:
Maria-Regina Kula, Heinrich Heine University of Düsseldorf, GERMANY
Oreste Ghisalba, Novartis Pharma AG, Basel, SWITZERAND

Investigation and on-line Analysis of Enantioselective Enzymatic Reactions
in Different Solvents via 2D-Fluorescence Spectroscopy
Torsten Knüttel, University of Hannover, GERMANY

Gas Phase Biotransformations Catalysed by Whole Cells
Isabelle Goubet, Université de la Rochelle, FRANCE

New Strategies for Enzyme Stabilization Involving the Protein Engineering
and the Immobilization in Mesoporous Materials
Haruo Takahashi, TOYOTA Central R&D Laboratories, Aichi, JAPAN

A Novel Transglutaminase from Bacillus subtilis Spores
Kenzo Yokozeki, Ajinomoto Co, JAPAN

Stabilization of NAD-dependent Formate Dehydrogenase from Candida
boidinii by Directed Evolution
Heike Slusarczyk, Heinrich Heine University of Düsseldorf, GERMANY

A Novel Molecularly Imprinted Catalyst Mimicking a Fructosyl Amine
Dehydrogenase for an Amperometric Sensor Constituent
Koji Sode, Tokyo University of Agriculture and Technology, JAPAN

Homology-independent Protein Engineering Using Incremental Truncation
Technology
Stefan Lutz, The Pennsylvania State University, USA

Creation of Protein/Enzyme Lineages with New Sequence Spaces by
Functional Salvage Screen
Hak-Sung Kim, Korea Advanced Institute of Science and Technology, Taejon, KOREA

Controlling Factors in Bacterial Cell-Free Protein Biosynthesis
Martin Siemann-Herzberg, University of Stuttgart, GERMANY-

Microchamber Arrays for PCR, Protein Synthesis and Cell Diagnosis
Eiichi Tamiya, Japan Advanced Institute of Science and Technology, Ishikawa, JAPAN

Development of Single-Molecule PCR Combined with Cell-Free Protein
Synthesis to Construct Large in-vitro Protein Library
Tsuneo Yamane, Nagoya University, JAPAN

Cell-Free Protein Expression: New Developments and Challenges
Erhard Fernholz, Roche Diagnostics, Penzberg, GERMANY

Cell Surface Engineering of Yeast - How to Apply It
Atsuo Tanaka, Kyoto University, JAPAN

16:30 - 17:00 Coffee Break

17:00 - 18.45 SESSION VII: ORAL PRESENTATION OF SELECTED POSTERS
Session Co-Chairs:
Harvey W. Blanch, University of California, Berkeley, USA
Hak-Sung Kim, Korea Advanced Institute of Science and Technology, Taejon, KOREA

17:00 - 17:10 Poster Chair: Introduction of Poster Prize Winners

17:10 - 17:30 TBA, Presentation of Poster (Second Prize)

17:30 - 17:50 TBA, Presentation of Poster (First Prize)

ENZYME ENGINEERING AWARD LECTURE
Session Chair:
Maria-Regina Kula, Heinrich Heine University of Düsseldorf, GERMANY

17:50 - 18:00 Maria-Regina Kula
Introduction of Enzyme Engineering Award Winner

18:00 - 18:45 2001 Enzyme Engineering Award Lecture
TBA

19:15 - 22.00 Banquet

Friday, October 12, 2001

06:30 - 10:00 Breakfast and Conference Adjournment

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POSTER SESSION I

Session Chair: Harvey W. Blanch, University of California, Berkeley, USA

1. Imaging Polarimetry for Biocatalysis Screening
Andreas Bommarius, Phillip Gibbs, Richard C. Wilson

2. Catalytic Antagonists: Selective degradation by Ligand-Targeted enzymes
Richard Bott, Benjamin G. Davis, David R.W. Hodgson, Astrid Ullman

3. Resolution of D.L-Carnitine Catalyzed by Snail Enzyme
Shu-gui Cao, Tian-Bing Ding, Gui-qiu Xie, Dong-Mei Lu

4. Maltose, a unique potential candidate for maltooligosaccharides synthesis by CGTase.
Didier Combes, Celine Monthieu, Alain Guibert

5. The South African advanced research centre of applied microbiology
Don A. Cowan, Stephanie Burton

6. An improved lipase bioconversion process for the production of fatty acid sugar esters
Jean-Mark Engasser, Francoise Chamouleau, Mohamed Ghoul

7. Thermostable glutamate dehydrogenase from a hyperthermophilic archaeon Pyrococcus horikoshii
Yan Feng, Shiyu Wang, Shugui Cao, Yoshitsugu Kosugi

8. Directed evolution of glucoamylase to increase thermostability and substrate specificity
Clark Ford, Yue Wang, Erica Fuchs, Roberto DaSilva

9. Application of Polylysine Tagging System to Purify and Immobilize Enzymes
Nam Soo Han, Dae-Hyuk Kweon, Chan Su Rha, Jin-Ho Seo

10. Exploiting microbial cell display for high-throughput screening of improved enzyme variants
Heung-Chae Jung, Soo-Keun Choi, Eui-Joong Kim, Su-Min Ko

11. Development of novel whole cell biocatalysts for production of biofuels and chemicals from biomass by using enzyme surface display systems
Akihiro Kondo, Hideki Fukuda, Mitsuyoshi Ueda, Atsuo Tanaka

12. A new approach to directed gene evolution by recombined extension on truncated templates (RETT)
Si Hyoung Lee, Eon Jung Ryu, Kyung Hwa Jung, Yong Chul Shin

13. Improvement of benzoylformate decarboxylase (BFD) for biocatalytic processes by directed evolution
B. Lingen, D. Kolter, M. Muller, M. Pohl

14. Gene Cloning, Expressing and Alkaline-tolerant Mechanism of Bacterial â-1,4-glycanases
Xiangmei Liu, Yinbo Qu, Meng Qi, Zhihong Wu, Jianqun Lin

15. Isolation of heat-stable deoxycytidine deaminase
Hidekatsu Maeda, Tsuyoshi Sakai, Hideo Mon-ma

16. The resistance of dextran modificed hyaluronidase against the heparin inhibition
Alexander V. Maksimenko, Yuliya Schechilina, Elena Tischenko

17. Modeling and Optimization of Directed Evolution Protocols for Enzyme Engineering
Gregory L. Moore, Costas D. Maranas

18. Improvements in Lipase Catalysed Hydrolisis of Racemic Ester of (RS)-Propanolol
R.V. Muralidhar, R.R. Chirumamilla, V.N. Ramachandran, R. Marchant and P. Nigam

19. In vitro engineering of Burkholderia cepacia KWI-56 lipase using PCR and cell-protein synthesis
Hideo Nakano, Junhao Yang, Koei Kobayashi, Tsuneo Yamane

20. Bi-directional improvement of oxidative and thermal stability of N-carbamyl-D-amino acid amydohydrolase by directed evolution
Ki-Hoon Oh, Sung-Hoon Nam, Hak-Sung Kim

21. Protein engineering of a â-1,3-Glucanase enzyme able to permabilize the yeast cell wall: mutagenetic studies of the catalytic center
A.Olivera, B.A. Andrews, J.A. Asenjo

22. Modeling and kinetic analysis of the reaction system using whole cells with separately-and co-expressed d-hydantoinase and N-Carbamoylase
Joo-Ho Park, Dong-Cheol Lee, Hak-Sung Kim

23. In Vitro selection of a (â/á)8-barrel scaffold using plasmid-linked display
Wayne M. Patrick, Jonathan M. Blackburn

24. An approach to have control of malolactic bacteria by Nisin fiber bioreactor
Paolo Spettoli, Anna Lante, Antonella Crapisi, Giovanna Lomolino

25. ASPD - a database and analysis tools for directly evolved biocatalysts
VP Valuev, DA Afonnikov, DA Grigorovich and MP Pomomarenko

26. Production and Preliminary Isolation of Trichoderma sp. T6 Xylanase
Ke Wu, Jingmin Cai, Renrui Pan, Jie Zhang, Bin Liu, Qianqian Wu, Benhong Yang

27. Evolutionary molecular engineering from Random polypeptides
Asao Yamauchi, Itaru urabe and Tetsuya Yomo

28. Molecular cloning of glucose- 3-dehydrogenase and its application for the synthesis of 3-keto derivatives of Saccharide
Tomohiko Yamazaki, Eri Akaike, Yuko Yamada, Yoshiko Sakata

29. The Hydrolysis of Ethyl Chrysanthemate by Lipase
Li-rong Yang, Jian-Ping Wu, Chi Zhang

30. Lipase-catalyzed kinetic resolution of allethrolone
Li-rong Yang, Jian-Ping Wu

31. Rational design of a more stable penicillin G. acylase
Sheng Yang, Haixu Tang, He Huang, Jiang Pan

32. Enhancement of the activity of alpha-aspartyl dipeptidase by Directed Evolution
Jin Zhang, Xiangdou Kong, Xiaojun Gou, Yiming Liu

33. High yield hybrid enzyme for fuel ethanol production
Jin Zhang, Xiangdou Kong, Xiaojun Gou, Bin Liu

34. In vitro selection of a new type of DNA-RNA complex nucleozyme that cleave RNA
Jin Zhang, Xiangdou Kong, Xiaojun Gou, Hongying Zhang

35. Study on the active sites of alpha-Aspastyl dipeptidase by Site-directed mutagenesis
Jin Zhang, Xiangdou Kong, Quan Zheng, Jun Lu

36. Chemo-Enzymatic Synthesis of RGD Diamide in Aqueous Water-Miscible
Xue-Zhong Zhang, Li-Qian Zhang, Yan-Dong Zhang, Li Xu

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POSTER SESSION II

Session Chair: Hak-Sung Kim, Korea Advanced Institute of Science and Technology, KOREA

1. Enhancement by Directed Evolution of the Thermostability and Catalytic Activity of a Newly Discovered D-Stereospecofic Amino-Acid Amidase from Ochrobactrum Anthropi (P2)
Yashuhisa Asano, Hidenobu Komeda, Naoyoshi Ishikawa

2. Thermostable exopolygalacturonate lyase from Bacillus licheniformis: cloning and properties of the cloned enzyme
S. Berensmeier, K. Buchholz, H. Diekmann, J. Meens

3. Isolation, characterization and inactivation kinetics of two thermostable amyloglucosidases
S. Berensmeier, P. Reilly and K. Buchholz

4. Biotransformation of phenolics using oxidative enzymes
S.G. Burton, A. Boshoff, D. Ryan, T. Luke

5. Sensitive method for determination of pectolytic activity with dilute pectin solution
Devlet Demirel, Ismail Hakki Boyaci, Mehmet Mutlu

6. Enzymatic synthesis of methyl a-D-Glucopyranoside acceptor-products using immobilized dextransucrases
A. Gomez de Segura, F.J. Plou, M. Alcalde, M. Remaud-Simeon

7. Lipase-catalyzed enantioselective esterification of ketoprofen in organic solvent
Yong Guo, Wei Du, Min-Hua Zong

8. Lipase-catalyzed enantioselective ammonolysis of phenylglycine methyl ester in organic solvent
Yong Guo, Wei Du, Min-Hua Zong

9. Quaternary structure engineering pyrroloquinoline quinone glucose dehydrogenase rational design and direct evolution of the enzyme for glucose sensor
Satoshi Igarashi, Koji Sode

10. Directed Evolution of a chitosanase for enhanced thermostability
Min Jung Kang, Chul Park, Si Hyoung Lee, Young Chul Shin

11. Lysozyme as a stabilizing agent for an enzyme biosensor for glucose and sucrose
N.G. Karanth, M.D. Gouda, M.A. Kumar, M.S. Thakur

12. Antibody catalysed reactions in microemulsion systems
F.N. Kolisis, E. Franqueville, H. Loutrari, F. Mellou

13. Solvent engineering instead of enzyme engineering: Ionic liquids as novel tool to enhance yield and enantioselectivity in enzyme catalysis
U. Kragl, N. Kaftzik, M. Eckstein, P. Wasserscheid

14. Production of Sialic Acid and CMP-Sialic Acid. Using Recombinant Escherichi coli as a Biocatalyst
Sun-Gu Lee, Kwany-Won Lee, Hyoung-Don Yun, Jung-Kyu Yi, Byung-Gee Kim

15. Multi-parameter biosensor in an automatic flow injection system for monitoring Kidney Dialysis Process
Gao Xiang Li, Jian Guo Liu and Li Chen Zhong

16. Efficient Electroenzymatic Oxidations by Reaction Engineering
Andreas Liese, Stephan Lütz, Iris Schröder

17. An artificial glutathione peroxidase with high catalytic efficiency: Selenoglutathione transferase
Guimin Luo, Xiajun Ren, Per Jemth, Bengt Mannervik

18. Lipase activation for intersterification by hydrocarbon-water interfaces
T. Maruyama, M. Nakajima, S. Ichikawa, M. Seki

19. Suppresion of the oxidation of polyunsaturated fatty acid by esterification with ascorbic acid through lipase-catalyzed condensation
Ryuichi Matsuno, Yoshiyuki Watanabe, Shuji Adachi

20. Effect of pressure on lipase-catalyzed esterification of stearic acid and hydrolysis of ethylstearate near the critical point in supercritical carbon dioxide
Osato Miyawaki, Hideki Nakaya, Kozo Nakamura

21. Biodegradation of Alkane by Psychrotropic Bacteria for Cold Environment
Yasutaka Morita, Hirotsugu Onishi, Sathuluri Ramachandra Rao, Toshifumi Sakaguchi, Yuji Murakami, Kenji Yokoyama, Eiichi Tamiya

22. Physical Complexation with polymers as a useful technique to activate enzymes in organic media
Yoshihiko Murakami, Akira Hirata

23. Rhizomucor miehei lipase: Differential Scanning Calorimetry and combined pressure/temperature stability studies in presence of soluble additives
Marilyne Noal, Eva Munoz, Monique Suderie, Didier Combes

24. A novel fungal enzyme, bilirubin dehydrogenase, useful for diagnostic bilirubin measurement
Jun Ogawa, Woro Triarsi, Hiromi Tanaka, Kenji Kano

25. Enzyme Crystals Encapsulated by Nanoengineered Polymer Multilayer for Bioreactors, Biosensors and Immunoassays
Reinhard Renneberg, D. Trau, W. Yang, M. Lehmann, F. Caruso

26. Enzymatic buffers enable positive-response inhibition-based biosensing of nerve agents
Alan J. Russell, Markus Erdelbinger, Joseph J. DeFrank, Geraldine Drevon

27. Electrochemical Enhancement of a Flow Amperometric Response for L-Ascorbate Using a Laccase-Column
Ikuo Satoh and Yasuhiro Iida

28. Whole Blood Neuropathy Target Esterase: Biomarker of Human Neuropathic Organophosphates Larisa V. Sigolaeva, Galina F. Makhaeva, Lyudmila V. Zhuravleva, Vladimier V. Malygin, Arkadi V. Eremenko, Alexander Makower, Frieder W. Scheller, Ilya N. Kurochkin

29. Enzymatic synthesis of palm based amino acid surfactants
El Soo, AB Salleh, M. Basri, CAN Razak

30. Enantioselective synthesis of (-)-methyl acetate using immobilised enzyme lipase in a recirculated packed bed reactor
Ng Tit Soo, Mohamad Roji

31. Energetics and Secondary Structural Changes of Enzymes after Covalent Modification
S. Srimathi, A. Yamuna, K.V. Jayanthi, P.V. Sundaram

32. Crystal structure of glycerol-3-phosphate acyltransferase from squash (Cucurbita moscata)
Taro Tamada, Michael D. Feese, Stefano R. Ferri, Yoichi Kato

33. Highly selective determination of acetylpolyamine
Kosuke Tomita, Shioka Sakurada, Satoshi Ota

34. Rational Modification of the Hydrogen Peroxide-Mediated Cytochrome C Inactivation Pathway
Brenda Valderrama, Humberto Garcia, Rafael Vasquez-Duhalt

35. Cleavage of Carbonates by Antibodies
Deljana Werner, Axel Warsinke, Jochen Woller, Bernd Kallies, Wolfgang Höhne, Burkhard Micheel

36. The Hydrolysis of Ethyl Chrysanthemate by Lipase
Jian-Ping Wu, Li-Rong Yang, Chi Zhang

37. Preliminary Study on Solid State Fermentation of Fucoidanase by Marine Fungus Dendryphiella arenaria N94
Qianqian Wu, Ke Wu, Jie Zhang, Bin Liu, Renrui Pan, Marinus Meiners, Song Qin, Jingmin Cai

38. The serine-297 of Escherichia coli Phenylalanine aminotransferase is crucial for catalytic efficiency
Szu-Pei Wu, Tzann-Shun Hwang, Wen-Chang Chang, Hsin Tsai

39. Preparation and autioxidative effects of corn peptides
Li Xu, Xianglu Li, Xiaoxia Wu, Xuezhong Zhang

40. The effect of salts on lipases activity in hexane
Li-rong Yang, Jian-Ping Wu, Shan-jing Yao

41. Immobilized recombinant penicillin acylase and synthesis of cephalexin
Zhong-Yi Yuan, Sheng Yang, Xin-Jia Wu, Ren-Bao Li

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