Posters

Cell Culture Engineering VII

April 1 - 6, 2002
Snowmass Conference Center
Snowmass Village, Colorado

Conference Co-Chairs:
Michael Betenbaugh
Johns Hopkins University, USA
John Aunins
Merck and Company, Inc. USA

United Engineering Foundation, Inc.
3 Park Avenue, 27th Floor
New York, NY 10016-5902
T: 1-212-591-7836 - F: 1-212-591-7441
engfnd@aol.com - www.engfnd.org

[ Poster Session II | Final Program ]

POSTER SESSION I
Tuesday-Wednesday, April 2-3, 2002

Stem Cells/Cell Therapies/ Tissue Engineering

I - 1. CD34 ANTIGEN EXPRESSION ON HEMATOPOETIC PROGENITOR CELLS IS RELATED TO EFFICIENCY OF MAGNETIC CELL SEPARATION
Kara E McCloskey, Georgia Institute of Technology, USA
Diane Leigh, Maciej Zborowski, Cleveland Clinic Foundation, USA
Jeffrey J. Chalmers, Ohio State University, USA

I - 2. KINETICS OF GENE EXPRESSION IN HUMAN T CELLS: EFFECTS OF REDUCED OXYGEN TENSION
Hadar Haddad, H. Yang, E. T. Papoutsakis, Northwestern University, USA

I - 3. SCALABLE BIOPROCESS FOR CONTROLLED DIFFERENTIATION OF EMBYRONIC STEM CELLS
Stephen Dang, Peter Zandstra, University of Toronto, CANADA

I - 4. RECEPTOR TRAFFICKING DYNAMICS OF HEMATOPOIETIC CELLS: A PREDICTIVE MODEL FOR CELL PROLIFERATION
Dominic C. Chow, E. Terry Papoutsakis, William M. Miller, Northwestern University, USA
Larissa A. Wenning, Merck & Co., USA

I - 5. AMINO ACID REQUIREMENT OF MURINE EMBRYONIC STEM CELLS DURING GROWTH AND DIFFERENTIATION
Andreas Werner, Jurgen Lehmann, University of Bielefeld, GERMANY

I - 6. MEMBRANE-MIMETIC SURFACE FOR HEMATOPOIETIC CELL CULTURE
Tor W. Jensen, Bi-Huang Hu, Phillip B. Messersmith, William M. Miller, Northwestern University, USA

I - 7. CONSTRUCTION AND EVALUATION OF THE LIVER FUNCTIONAL HEPG2 CELL LINE FOR HYBRID BIOARTIFICIAL LIVER SUPPORT SYSTEM
Takeshi Omasa, Kazumi Kim, Shin-ya Hiramatsu, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga, Kiichi Fukui, Osaka University, JAPAN
Shin Enosawa, National Children's Medical Research Center, JAPAN

I - 8. THE IMPACT OF PROCESS CONDITIONS ON A SMOOTH MUSCLE CELL LINE FOR USE IN A TISSUE ENGINEERED BLOOD VESSEL
Julia Markusen, Chris Mason, Susana Levy, Parviz Ayazi-Shamlou, Peter Dunnill, University College London, UNITED KINGDOM

I - 9. THE ROLE OF SONIC HEDGEHOG IN ADULT NEURAL PROGENITOR CELL PROLIFERATION IN VITRO AND IN VIVO
Karen Lai, David V. Schaffer, University of California at Berkeley, USA
Brian Kaspar, Fred H. Gage, Salk Institute for Biological Studies, USA

I - 10. IN VIVO GENERATION OF THREE DIMENSIONAL VASCULARIZED TISSUE
Aurora Messina, Rejhan Idrizi, Oliver C.S. Cassell, Wayne A. Morrison, Victorian Tissue Engineering Centre (VTEC), AUSTRALIA

Differentiation and Death

I - 11. GADD153 AS A PROGNOSTIC INDICATOR OF APOPTOSIS IN CHO AND NS0 CELLS
Alan J Dickson, Idsada Lengwehasatit, Tracy Murphy, University of Manchester, UNITED KINGDOM
Nigel Woods, British Biotech, UNITED KINGDOM

I - 12. INHIBITION OF PROLIFERATION BY AN EXCRETED, TRUNCATED FORM OF HISTONE H4
Lena Häggström, Eva Bertram, Royal Institute of Technology, SWEDEN
Karin Calles, KaroBio AB, SWEDEN
Jenny Hassel, Fritz Schweikhart, AstraZeneca Biotech Laboratory, SWEDEN

I - 13. REPEATED PATTERNS OF CYTOKINE CONCENTRATION AND INTERACTION DEPENDENCIES SIMPLIFY ANALYSIS OF HEMATOPOIETIC TOTAL, PROGENITOR AND STEM CELL POPULATION EXPANSIONS
James M. Piret, Julie Audet, Muhammad A. S. Chaudhry, Bruce D. Bowen, Connie J. Eaves, University of British Columbia, CANADA

I - 14. A POSSIBLE ROLE FOR THE TOXIC METABOLITE METHYLGLYOXAL IN THE SIGNALING PATHWAYS ASSOCIATED WITH NECROTIC CELL DEATH.
Frank Chaplen, Oregon State University, USA

I - 15. EFFECT OF THE OVEREXPRESSION OF ANTIAPOPTOTIC VIRAL PROTEINS HOMOLOGUES OF BCL-2 ON SURVIVAL OF HYBRIDOMA CELLS
Francesc Gòdia, Joaquim Vives, Sandra Juanola, Jordi J. Cairo, Universitat Autònoma de Barcelona, SPAIN
Eva Prats, Lluis Cornudella, IBMB-CSIC, SPAIN

I - 16. DO CHO CELLS COMMIT HARAKIRI?
Lena Häggström, Royal Institute of Technology, SWEDEN
Nathalie Chatzissavidou, Ioannis Tukulekas, Biovitrum AB, SWEDEN

I - 17. MOLECULAR MODULATION OF CELL CYCLE AND APOPTOSIS FOR ENHANCEMENT OF PRODUCTIVITY IN MAMMALIAN CELLCULTURE
Mohamed Al-Rubeai, University of Birmingham, UNITED KINGDOM

I - 18. APOPTOSIS AND ITS SUPPRESSION IN HEPATOCYTES
Mohamed Al-Rubeai, Nyaradzo T. Mukwena, University of Birmingham, UNITED KINGDOM

Gene Expression

I - 19. A VERSATILE EXPRESSION SYSTEM FOR RAPID AND STABLE PRODUCTION OF RECOMBINANT PROTEINS
Myung-Sam Cho, H. Yee, C. Brown, B. Mei, C. Mirenda, S. Chan, Bayer Biotechnology, USA

I - 20. IMPROVING HUMAN THERAPY BY ARTIFICIAL REGULATORY NETWORKS
Martin Fussenegger, Cornelia Fux, Jens Kelm, Barbara Mitta, Wilfried Weber, ETH Institute of Biotechnology, SWITZERLAND

I - 21. NEW METHODS FOR IMPROVED MAMMALIAN EXPRESSION OF RECOMBINANT PROTEINS
Arnold H. Horwitz, XOMA (US) LLC, USA

I - 22. OPTIMIZATION OF GENETIC CONTROL NETWORK OF AN INDUCIBLE OVEREXPRESSION
SYSTEM
Dhinakar S. Kompala, Mark C. Mowry, Matthew L. Lipscomb, Lorinda Girouard, University of Colorado, USA

I - 23. KINETIC MODEL OF BIP- AND PDI-MEDIATED PROTEIN FOLDING AND ASSEMBLY
Barbara Andrews, D. Sepulveda, P. Venegas, R. Caviedes, P. Caviedes, J.A. Asenjo, University of Chile, CHILE

I - 24. APPLICATIONS OF THE HEK.EBNA EXPRESSION SYSTEM: MINI-, MAXI-, MULTIPARALLEL
Sabine Geisse, Klaus Memmert, Rita Schmitz, Eric Weber, Mauro Zurini, Novartis Pharma Research, SWITZERLAND

I - 25. EFFECT OF DOXYCYCLIN-REGULATED ERP57 EXPRESSION ON THROMBOPOIETIN PRODUCTIVITY IN RECOMBINANT CHO CELLS
Gyun Min Lee, Sun Ok Hwang, Joo Young Chung, KAIST, KOREA

I - 26. CHARACTERISATION OF THE STABILITY OF RECOMBINANT PROTEIN PRODUCTION IN THE GS-NS0 EXPRESSION SYSTEM
Louise Barnes, Alan Dickson, University of Manchester, UNITED KINGDOM
Catherine Bentley, Nicola Moy, GlaxoSmithKline, UNITED KINGDOM

I - 27. E.REX - ERYTHROMYCIN-REGULATED EXPRESSION A NEW DEGREE OF FREEDOM IN ADJUSTABLE MAMMALIAN GENE REGULATION
Wilfried Weber, Beat P. Kramer, Cornelia Fux, Rene R. Marty, Martin Fussenegger, ETH Institute of Biotechnology, SWITZERLAND

I - 28. PRODUCTION OF RECOMBINANT ENDOSTATIN FROM STABLY TRANSFORMED LEPIDOPTERAN AND DIPTERAN INSECT CELLS
In Sik Chung, Kyung Hee University, KOREA

I - 29. RECOMBINANT PROTEIN PRODUCTION WITH VACCINIA VIRUS
Nicole A. Bleckwenn, William E. Bentley, Joseph Shiloach, LCDB, NIDDK, NIH / Center for Agricultural Biotechnology, USA

I - 30. TRANSIENT GENE EXPRESSION AT THE 100 LITER SCALE
Philippe Girard, Madiha Derouazi, Martin Jordan, Michaela Bourgeois, Florian M. Wurm, Swiss Federal Institute of Technology, SWITZERLAND
Barbara Jacko, BioWhittaker Inc., SWITZERLAND

I - 31. DEVELOPING HIGH-PRODUCING CHINESE HAMSTER OVARY CELL LINES BY FLP RECOMBINASE ASSISTED SITE-SPECIFIC GENE INTEGRATION
N. Lin, S. Konchar, T. Garringer, A. Lagu, N. Jenkins, C. L. Hershberger, Lilly Research Labs, USA

I - 32. HUMAN C-FOS PROMOTER MEDIATES HIGH-LEVEL GENE EXPRESSION: APPLICATION IN MONITORING THE DYNAMIC EXPRESSION OF GFP IN DIFFERENT MAMMALIAN CELL LINES
Jingxiu Bi, Petra Buhr, An-Ping Zeng, Wolf-Dieter Deckwer, Manfred Wirth, GBF- German Research Center for Biotechnology, GERMANY

I - 33. PRODUCTION OF HIGH EXPRESSING, STABLE CELL LINES USING GPEX™ TECHNOLOGY
Dona York, Robert D. Bremel, Gala Biotech, USA

I - 34. A COMPARISON OF PROMOTERS USED FOR TRANSIENT EXPRESSION OF RECOMBINANT PROTEIN IN CHO CELLS
Mimi Liao, Peter Gray, Noelle Sunstrom, University of New South Wales, AUSTRALIA

I - 35. TRANSIENT GENE EXPRESSION IN SUSPENSION HEK-293 CELLS: OPTIMIZATION OF A CALCIUM PHOSPHATE BASED METHOD FOR LARGE-SCALE APPLICATIONS
Lucia Baldi, Raymond Jacquet, Martin Jordan, Philippe Girard, Keyvan Iglesias, Elisabeth Derow, Sylviane Picasso, Floria Wurm, Swiss Federal Institute of Technology, SWITZERLAND

I - 36. MULTIGENE ENGINEERING OF MRNA TRANSLATION INITIATION IN EUKARYOTIC CELLS FOR ENHANCED RECOMBINANT PROTEIN PRODUCTION
Michele Underhill, David James, University of Kent, UNITED KINGDOM
John Birch, Robert Kallmeier, Lonza Biologics plc, UNITED KINGDOM
Christopher Proud, University of Dundee, UNITED KINGDOM

I - 37. SUSTAINED, HIGH-YIELD PRODUCTION OF RECOMBINANT PROTEINS IN TRANSIENTLY-TRANSFECTED COS-7 CELLS
James Varani, Randall N. Knibbs, Melissa R. Allen, Lloyd Stoolman, University of Michigan, USA
William J. Hillegas, SoloHill Engineering, USA

I - 38. SELECTION OF RECOMBINANT CELL LINES: DEVELOPMENT OF AN INTEGRATED STRATEGY
Adolfo Castillo Vitlloch, Evelyn Faife, Kathya R. de la Luz, Svieta Victores, Center of Molecular Immunology, CUBA

I - 39. THE CUMATE GENE-SWITCH: A NEW INDUCIBLE SYSTEM FOR REGULATED EXPRESSION IN MAMMALIAN CELLS
Bernard Massie, Alaka Mullick, Rene Warren, Maria Koutroumanis, Felix Malenfant, Linda Lamoureux, Isabel Boivin, Peter Lau, Institut de recherche en biotechnologie-CNRC, CANADA

I - 40. EFFICIENT SELECTION OF MONOCLONAL, ANTIGEN-SPECIFIC AND HIGH-PRODUCING HYBRIDOMA CELL LINES IN A SINGLE CLONING STEP BY FLOW CYTOMETRY AND CELL SORTING
Ernst Böhm, Nicole Borth, Gabriele Stiegler, Rainer Hahn, Boris Ferko, Renate Kunert, Hermann Katinger, University of Agricultural Sciences, AUSTRIA

I - 41. TRANSIENT TRANSFECTION IN BIOREACTORS: EFFECT OF PLASMID QUALITY AND CONTAMINANTS ON PROTEIN EXPRESSION
Georg Schmid, E.-J. Schlaeger, B. Wipf, Hoffmann-La Roche AG, SWITZERLAND

I - 42. THE ANALYSIS OF RECOMBINANT-PROTEIN SECRETION IN CHINESE HAMSTER OVARY CELLS USING GENOMIC AND PROTEOMIC TECHNOLOGIES
Eric J. Hayduk, Kelvin H. Lee, Cornell University, USA

Quality

I - 43. SCREENING FOR ADDITIONAL CELL PROPERTIES: SELECTION OF SUBCLONES WITH ALTERED PRODUCTION KINETICS OR IMPROVED STABILITY BY CELL SORTING USING AN AFFINITY MATRIX SECRETION ASSAY
Nicole Borth, Ernst Bohm, Regina Voglauer, Renate Kunert, Willi Steinfellner, Hermann Katinger, University of Agricultural Sciences, AUSTRIA

I - 44. DEVELOPMENT OF AN HPLC BASED ASSAY TO ANALYZE SIALYLATION PATTERNS OF RECOMBINANT PROTEINS PRODUCED DURING MEDIUM OPTIMIZATION PROGRAMS FOR CHINESE HAMSTER OVARY (CHO) CELLS
Tom Fletcher, William Jay Treat, Yoichi Oba, Irvine Scientific, USA

I - 45. PRODUCTION OF A SIALYLATED RECOMBINANT GLYCOPROTEIN IN A TRICHOPLUSIA NI INSECT CELL LINE
Christoph E. Joosten, Michael L. Shuler, Cornell University, USA

I - 46. A NOVEL INSECT CELL LINE IS CAPABLE OF SIALYLATION OF A RECOMBINANT PROTEIN
Laura A. Palomares, Universidad Nacional Autonoma de Mexico, MEXICO
Christoph E. Joosten, Patrick Hughes, Robert R. Granados, Michael L. Shuler, Cornell University, USA

I - 47. CHARACTERIZATION OF THE GLYCOSYLATION PATTERN OF A SECRETED REPORTER PROTEIN FROM AN INDUCIBLE EXPRESSION SYSTEM IN CHO CELLS
Matthew L. Lipscomb, Brad Bendiak, Dhinakar S. Kompala, University of Colorado at Boulder, USA

I - 48. TAILORING BIOLOGICAL ACTIVITY OF ANTIBODIES WITH GLYCOSYLATION ENGINEERING
Stefanie Weikert, John Briggs, Rod Keck, Bob Shields, Lynne Krummen, Genentech, Inc., USA

I - 49. ENGINEERING OF SIALYLATION PATHWAY IN INSECT CELLS
Karthik Viswanathan, Shawn Lawrence, Y.C. Lee, Michael Betenbaugh, Johns Hopkins University, USA
Stephan Hinderlich, Freie Universitat Berlin, GERMANY

I - 50. N-GLYCAN SITE OCCUPANCY PROCESSING IN MAMMALIAN AND INSECT CELLS
Jullian Jones, Karthik Viswanathan, Sharon Krag, Michael Betenbaugh, Johns Hopkins University, USA

I - 51. GLYCOENGINEERING OF HUMAN CELL LINES
H. Baumeister, M. Schlangstedt, U. Schober, H. Tandara, S. Goletz, Nemod Immuntherapie AG, GERMANY

Genomics and Proteomics

I - 52. ANALYSIS OF THE MAMMALIAN NS0 CELL PROTEOME
Elizabeth. A. Sage, C. Mark Smales, David C. James, University of Kent at Canterbury, UNITED KINGDOM
Carol T. Marshall, GlaxoSmithKline, UNITED KINGDOM

I - 53. PROTEOMIC ANALYSIS OF AN INDUCIBLE EXPRESSION SYSTEM IN RECOMBINANT CHINESE HAMSTER OVARY CELLS
Lorinda G. Girouard, Dhinakar S. Kompala, University of Colorado, USA

I - 54. GENOMIC ANALYSIS OF AN INDUCIBLE EXPRESSION SYSTEM IN RECOMBINANT CHINESE HAMSTER OVARY CELLS
Mark C. Mowry, Dhinakar S. Kompala, University of Colorado at Boulder, USA

I - 55. GENOMIC AND PROTEOMIC ANALYSIS OF METABOLIC SHIFT IN CONTINUOUS CELL CULTURE
Marcela de Leon Gatti, Rashmi Korke, Wei-Shou Hu, University of Minnesota, USA
Anette Rink, Craig Beattie, University of Nevada, USA
Teck Keong Seow, Maxey Chung, The National University of Singapore, SINGAPORE

I - 56. THE FUNCTIONAL COMPETENCE OF ANIMAL CELLS IN CULTURE: ANALYSIS OF THE SECRETORY PATHWAY PROTEOME
Daniel E. Alete, C. Mark Smales, Peter Klappa, David C. James, University of Kent at Canterbury, UNITED KINGDOM
John R. Birch, Lonza Biologics plc, UNITED KINGDOM

I - 57. THE FUNCTIONAL COMPETENCE OF ANIMAL CELLS IN CULTURE: ANALYSIS OF THE CELLULAR PROTEOME
C. Mark Smales, Elizabeth A. Sage, Daniel E. Alete, Peter Klappa, David C. James, University of Kent at Canterbury, UNITED KINGDOM
Michael J. Dunn, University of London, UNITED KINGDOM
Carol T. Marshall, GlaxoSmithKline, UNITED KINGDOM
John R. Birch, Andrew Racher, Lonza Biologics plc, UNITED KINGDOM

I - 58. ANALYSIS OF THE MOUSE NS0 GENOME
M. Al-Rubeai, University of Birmingham, UNITED KINGDOM
Z. Tymowska-Lalanne, P. Underhill, MRC, UNITED KINGDOM

Viral Vectors/Production

I - 59. MICROCARRIER CELL CULTURE PROCESS FOR PRODUCING RETROVIRUS AND ADENOVIRUS VECTORS
Suh-Chin Wu, Gloria Yia-Ling Huang, National Tsing Hua University, TAIWAN
Jin-Hwang Liu, Veterans General Hospital-Taipei, TAIWAN

I - 60. PRODUCTION OF DELETED ADENOVIRAL VECTORS FOR GENE THERAPY
Tammy Malone, Jennifer Sullivan, Crystal Auger, Abraham Scaria, Samual Wadsworth, Genzyme Corporation, USA

I - 61. DEVELOPMENT OF AN ADENOVIRUS BASED VETERINARY ORAL VACCINE
Paula M. Alves, Delia Goncalves, Cristina C. Peixoto, Pedro E. Cruz, Manuel J. T. Carrondo, IBET/ITQB, PORTUGAL

I - 62. EFFECT OF CULTURE CONDITIONS AND MEDIUM COMPOSITION UPON RETROVIRAL VECTORS STABILITY AND PHYSICAL-CHEMICAL PROPERTIES
Pedro E. Cruz, Delia Goncalves, Miguel Teixeira, Manuel J. T. Carrondo, IBET/ITQB, PORTUGAL

I - 63. ELEVATED PH INCREASES POLYMER-AIDED RETROVIRAL VECTOR TRANSDUCTION EFFICIENCY
Yong Chen, Tor W. Jensen, William M. Miller, Northwestern University, USA

I - 64. THE SELECTION OF BACULOVIRUS FEW POLYHEDRA MUTANTS IN INSECT CELL CULTURE IS AFFECTED BY THE TIME OF HARVEST FOR BUDDED VIRUS
Linda H. L. Lua, Steven Reid, The University of Queensland, AUSTRALIA

I - 65. INFLUENCE OF THE SUGAR SOURCE ON GROWTH AND PRODUCTION KINETICS OF RETROVIRAL VECTOR PRODUCING CELLS
C. Fiamma-Geny, C. Rochette, L. Millot, O. Danos, O. W. Merten, Genethon III , FRANCE

I - 66. OPTIMIZING HARVEST TIME FOR BACULOVIRUS AMPLIFICATIONS
Ana M. Carrion, Ziggy Demyt, Jorege Godoy, Geoff Hodge, Millennium Pharmaceuticals, Inc., USA

I - 67. RECOMBINANT-LACTADHERIN EXPRESSION IN TWO INSECT CELL LINES: STUDY OF THE EFFECT OF MOI AND TOI ON THEIR GLYCAN MOIETIES
Sandino Estrada, Octavio T. Ramirez, National Autonomous University of Mexico, MEXICO

I - 68. CHALLENGES IN THE DESIGN AND PRODUCTION OF FIRST AND THIRD GENERATION ADENOVIRUS VECTORS FOR GENE THERAPY
H. Manosalva, E. Karahanian, Y. Israel, B. A. Andrews, J. A. Asenjo, University of Chile, CHILE

I - 69. BACULOVIRUS MEDIATED GENE DELIVERY IN MAMMALIAN CELLS
Thomas A. Kost, J. Patrick Condreay, Sam Witherspoon, William Clay, Ray Merrihew, Jean-Louis Klein, GlaxoSmithKline, USA

I - 70. ADENOVIRUS-BASED LIBRARIES: EFFICIENT GENERATION OF RECOMBINANT ADENOVIRUSES BY POSITIVE SELECTION WITH THE ADENOVIRUS PROTEASE
Bernard Massie, Seyyed Mehdy Elahi, Wahiba Oualikene, Lili Naghdi, David Gagnon, Maureen O'Connor-McCourt, Biotechnology Research Institute-CNRC, CANADA

I - 71. INDUCIBLE EXPRESSION OF E1B19K IN A SERUM-FREE ADAPTED NSO CLONE TRANSIENTLY IMPROVED VIABILITY YET DID NOT IMPROVE RECOMBINANT ANTIBODY PRODUCTION IN A PERFUSION SYSTEM
Bernard Massie, Patrick Daoust, Lucie Bourget, Antoine Caron, Amine Kamen, Biotechnology Research Institute-CNRC, CANADA
Mario Jolicoeur, Ecole Polytechnique de Montreal, CANADA

I - 72. THE IMPACT OF PROCESS CONDITIONS ON THE CENTRIFUGAL RECOVERY OF A DISABLED HERPES SIMPLEX VIRUS
P. Lotfian, M. S. Levy, R. S. Coffin, P. Ayazi Shamlou, University College, London, UNITED KINGDOM

I - 73. PH, PCO2 AND TEMPERATURE EFFECT ON R-ADENOVIRUS PRODUCTION
A. Garnier, M. Jardon, Laval University, CANADA

I - 74. PRODUCTION OF RECOMBINANT ADENOVIRUS BY 293S CELLS CULTURED IN PERFUSION
A. Garnier, V. Cortin, Laval University, CANADA
J. Thibault, University of Ottawa, CANADA
D. Jacob, BRI-NRC, CANADA

I - 75. EFFECTIVE MONITORING OF ADENOVIRUS INFECTION KINETICS USING FLOW CHEMISTRY
Marie-Pierre Gentile, Weichang Zhou, Merck Research Laboratories, USA

I - 76. OPTIMIZATION OF A 293 SUSPENSION PROCESS FOR ADENOVIRUS PRODUCTION
Isabelle Nadeau, Gabriel Seanez, Florence Wu, BioReliance Corp., USA

I - 77. DEVELOPMENT OF MRC-5 CELL CULTURE PROCESS TO PRODUCE SMALLPOX VACCINE
Florence Wu, Isabelle Nadeau, Kesav Reddy, Karine Reiter, Shantha Kodihalli, John Gilly, BioReliance Corp., USA
David Clanton, DynPort Vaccine Co., USA

[ Poster Session I | Final Program ]

POSTER SESSION II
Thursday-Friday, April 3-4, 2002

Mabs

II - 1. CULTURE OF HYBRIDOMA CELLS UNDER OSCILLATING DISSOLVED OXYGEN TENSION: IS IT CRITICAL TO MAINTAIN HOMOGENEOUS CONDITIONS?
Octavio T. Ramírez, Jose A. Serrato, National University of Mexico, MEXICO

II - 2. ANAYLSIS OF INSTABILITY IN CELL LINES EXPRESSING IGG
Lekan Daramola, Wyn Forrest-Owen, Diane Hatton, Ray Field, Cambridge Antibody Technology, USA

II - 3. IDEC CELL CULTURE MANUFACTURING PLATFORM ENABLES FASTER DEVELOPMENT OF COST-EFFECTIVE COMMERCIAL PRODUCTION FOR MONOCLONAL ANTIBODIES
David Y. H. Chang, Andrew Grant, Wolfgang Noe, IDEC Pharmaceuticals Corporation, USA

II - 4. ENHANCEMENT OF MONOCLONAL ANTIBODY PRODUCTION BY SYNTHETIC OLIGOPEPTIDES
Frantisek Franek, Institute of Experimental Botany, CZECH REPUBLIC
Tomas Eckschlager, Charles University, CZECH REPUBLIC
Hermann Katinger, University of Agricultural Sciences, AUSTRIA

II - 5. PROCESS SCALABILITY THROUGHOUT THE CLINICAL DEVELOPMENT OF RECOMBINANT CHO-DERIVED MONOCLONAL ANTIBODY THERAPEUTICS
Eric Fallon, Paul Garza, Andrew Grant, Wolfgang Noe, IDEC Pharmaceuticals Corporation, USA

II - 6. IMPACT OF PROCESS CHANGES ON HUMANIZED ANTIBODY CHARACTERISTICS
Mark P. Backer, P. Motchnik, L. Dumont, Protein Design Labs, Inc., USA

II - 7. THE ADAPTATION AND DEVELOPMENT OF A GLUTAMINE INDEPENDENT NSO HOST CELL LINE FOR THE PRODUCTION OF HUMANIZED ANTIBODIES
John E. Burky, Paul Sauer, Sharyn Farnsworth, Taymar Hartman, Protein Design Labs, Inc., USA

II - 8. RAPID METABOLIC SCREENING FOR USE IN IMPROVEMENT OF HYBRIDOMA CELL CULTURE PROCESSES
Lindsey Clark, R. Robert Balcarcel, Vanderbilt University, USA

II - 9. OPTIMISATION OF MONOCLONAL ANTIBODY PRODUCTION IN PERFUSION BIOREACTOR WITH ACOUSTIC SEPARATION DEVICE
M.C.F. Dalm, D.E. Martens, J. Tramper, Wageningen University, THE NETHERLANDS
W.M.J. van Grunsven, Diosynth BV, THE NETHERLANDS
A. Oudshoorn, Applisens BV, THE NETHERLANDS

II - 10. PER.C6TM AS A PLATFORM FOR MANUFACTURING MONOCLONAL ANTIBODIES
Bram Bout, Crucell Holland NV, THE NETHERLANDS

Physiology and Metabolism

II - 11. ALTERED GROWTH FACTOR SIGNALING INVOLVED IN SERUM-FREE ADAPTATION OF CHO CELLS
Pranhitha Reddy, Brian Rasmussen, Raymond Davis, Teri Aldrich, Arvia Morris, Immunex Corp., USA

II - 12. THE RESPONSE OF GS-NS0 MYELOMA CELLS TO SINGLE AND MULTIPLE PH PERTURBATIONS
Jason J Osman, John Birch, Lonza Biologics, UNITED KINGDOM
Julie Varley, Imperial College, UNITED KINGDOM

II - 13. HIGH-THROUGHPUT METABOLIC FLUX ANALYSIS
R. Robert Balcarcel, Lindsey M. Clark, Yuansheng Yang, Vanderbilt University, USA

II - 14. IMPROVING GLUCOSE AND GLUTAMINE METABOLISM OF HUMAN HEK 293 AND TRICHOPLUSIA NI INSECT CELLS ENGINEERED TO EXPRESS A CYTOSOLIC PYRUVATE CARBOXYLASE ENZYME
Cynthia B. Elias, Eric Carpentier, Yves Durocher, Loius Bisson, Amine A. Kamen, Biotechnology Research Institute-CNRC, CANADA

II - 15. OVER-EXPRESSION OF NHE1 IN CHO CELLS ENHANCES RESISTANCE TO ELEVATED AMMONIA CONCENTRATIONS
Lisa R. Abston, William M. Miller, Northwestern University, USA

II - 16. ENHANCED EXPRESSION OF GLYCOSYLATED ERYTHROPOIETIN FROM CHO CELLS BY TWO ALKANOIC ACIDS
Young O. Baek, D.K. An, S.O. Lee, J.H. Kim, Cheiljedang Corporation, KOREA
B.H. Chun, Jeil, Biotechservices, Inc., KOREA

II - 17. EFFECT OF METAL IONS ON THE PRODUCTION OF B-DOMAIN DELETED BLOOD COAGULATION FACTOR VIII IN A PROTEIN-FREE CHO CELL CULTURE
Bokhwan Chun, Namhyun Chung, Won-Gi Bang, Korea University, KOREA
Taeho Byun, Green Cross Corp., KOREA

II - 18. RECEPTOR EXPRESSION AND EFFECTS OF INSULIN AND LONG™R3 INSULIN-LIKE GROWTH FACTOR-I ON CHINESE HAMSTER OVARY CELLS
Catherine A. Yandell, B.J. Wade, A.P. Simula, C. Goddard, GroPep Limited, AUSTRALIA

II - 19. ALTERATION OF CELLULAR METABOLISM OF CHINESE HAMSTER OVARY CELLS DURING FED-BATCH CULTURES
Qin Meng, Jongchan Lee, Patrick Hossler, Wei-Shou Hu, University of Minnesota, USA

II - 20. FACTORS FOR THE OPTIMIZATION OF THE CULTURE OF NEURONAL CELL LINES FOR THE DEVELOPMENT OF CELL TRANSPLANT MATERIAL
B.A. Andrews, D. Sepulveda, P. Venegas, R. Caviedes, P. Caviedes, J.A. Asenjo, University of Chile, CHILE

II - 21. THE EFFECT OF SUGAR CONSUMPTION RATE ON THE GLYCOSYLATION IN ANTITHROMBIN-III PRODUCTION
Takeshi Omasa, Osaka University, JAPAN

II - 22. IMAGING AND QUANTITATIVE ANALYSIS OF PHOSPHOINOSITIDE 3-KINASE SIGNALING IN LIVING CELLS: IMPLICATIONS FOR DIRECTED CELL MIGRATION AND WOUND HEALING
Jason Haugh, Ian Schneider, North Carolina State University, USA

II - 23. ENHANCED PROTECTION AGAINST APOPTOSIS USING MODIFIED ANTI-APOPTOSIS GENES
Bruno Figueroa Jr., Tina M. Sauerwald, J. Marie Hardwick, Michael J. Betenbaugh, Johns Hopkins University, USA
George A. Oyler, University of Maryland

Media

II - 24. DEVELOPMENT OF A PROTEIN-FREE BIOREACTOR PRODUCTION PROCESS USING A HUMAN CELL HOST
Charles A. Sardonini, Carla Alpert, Christopher Francis, Kenneth DeRosier, Antoinette Zuliani, Transkaryotic Therapies, Inc., USA

II - 25. OPTIMIZING AN INSECT CELL FED-BATCH CULTURE MEDIUM USING A GENETIC ALGORITHM
Rolf Marteijn, Otto Jurrius, Joris Dhont, Dirk Martens, Hans Tramper, Wageningen University, THE NETHERLANDS

II - 26. OPTIMIZING MONOCLONAL ANTIBODY PRODUCTION IN SERUM-FREE MEDIA USING DEFINED CULTURE PROCESSES
Brandon L. Pence, Bill B. Barnett, Stefan A. Weiss, HyClone Laboratories, Inc., USA

II - 27. OPTIMIZED NUTRIENT ADDITIVES FOR FED-BATCH CULTURE
Stephen F. Gorfien, William Paul, David W. Jayme, Invitrogen Corporation, USA

II - 28. INFLUENCE OF CELL CULTURE MEDIA ON ADHESION OF ANIMAL CELLS ON MICROCARRIERS
Karlheinz Landauer, Susanne Wiederkum, Markus Durrschmid, Otto Doblhoff-Dier, Institute of Applied Microbiology, AUSTRIA
Gerald Bluml, Amersham Biosciences, AUSTRIA

II - 29. REPLACEMENT OF WHEAT PEPTONE DURING DEVELOPMENT OF A DEFINED MEDIUM FOR RECOMBINANT SP2/0 CELLS
Michael C. Borys, Katherine Hughes, Jon M. Ryan, Abbott Laboratories, USA

II - 30. SPODOPTERA FRUGIPERDA (SF-9) CELL GROWTH AND LONGEVITY IS PROMOTED BY SUPPLEMENTATION OF CULTURE MEDIUM WITH HEMOLYMPH OF LONOMIA OBLIQUA
Luis Maranga, Cristina C. Peixoto, Manuel J.T. Carrondo, IBET/ITQB, PORTUGAL
Ronaldo Z. Mendonca, Roberto H. P. Moraes, Instituto Butantan, BRAZIL

II - 31. SCREENING OF SOYBEAN HYDROLYSATES USED IN PRODUCTION OF THERAPEUTIC ANTIBODIES EXPRESSED IN SP2/0 AND NSO CELL LINES
Daniel Velez, Jose Santiago, Maya Grosh, Lori DelMauro, Girish Pendse, ImClone Systems Incorporated, USA

II - 32. APPLICATION OF NEW PROTEIN-FREE ANIMAL COMPONENT-FREE MEDIUM FOR ADHERENT CHO CELL RECOMBINANT PROTEIN EXPRESSION SYSTEMS
A. Albee, J.S. Ross, D.M. Goodnight, K. Kao, M.V. Caple, Sigma-Aldrich Corporation, USA

II - 33. EFFICIENT DELIVERY OF LIPIDS TO HYBRIDOMA CULTURE BY USE OF NOVEL GRANULATED DRY-FORM MEDIUM TECHNOLOGY
David Jayme, Richard Fike, Barbara Dadey, Richard Hassett, Jennifer Walowitz, GIBCO/Invitrogen, Inc., USA

II - 34. DEVELOPMENT AND OPTIMIZATION OF A PROTEIN-FREE, ANIMAL COMPONENT FREE CHO MEDIA USING "DIRECTED" FACTORIAL DESIGN
Chung Chun, Thomas Ryll, John D. Chung, Abgenix, USA

II - 35. A NEW SERUM-FREE SUSPENSION GROWTH MEDIUM FOR THE HEK 293 CELL LINE
Shelley Wilcox, Manisha Sahni, Sarah L. Gilliland, Douglas R. Purtle, Karen J. Etchberger, JRH Biosciences, USA

II - 36. TRANSFECTION OF MULTIPLE SERUM-FREE ADAPTED CHINESE HAMSTER
Shelley Wilcox, Robert C. Heckathorn, Sandy McNorton, Karen J. Etchberger, JRH Biosciences, Inc., USA

II - 37. EFFECT OF DMSO ON INTRACELLULAR PHYSIOLOGY
Wai Lam W. Ling, Liang Deng, Schering-Plough Research Institute, USA

II - 38. AMINO ACID METABOLISM AND PRODUCTIVITY OF CHO CELLS IN A HOMOGENEOUS CONTINUOUS PERFUSION CULTURE USING AN ULTRASONIC RETENTION DEVICE IN VARIED CONTROLLED CONDITIONS
John Crowley, Carina Schlukebir, Jose M. Coco Martin, DSM Biologics, NETHERLANDS
Stefaine Hubmann, Andreas Herrmann, Cardion AG, NETHERLANDS

Optimization/Process/Scale-up/Monitoring

II - 39. OPTIMISING RECOMBINANT PROTEIN EXPRESSION FROM CHO CELLS BY MANIPULATION OF MULTIPLE FACTORS
Gary Pettman, Sam Byerley, GlaxoSmithKline, USA

II - 40. CONTINUOUS AND SCALABLE PRODUCTION OF A FILTERED PRODUCT FROM A BIOREACTOR
Jim Furey, Jerry Shevitz, Refine Technology, Co., USA

II - 41. IMPROVED FERMENTATION PROCESSES FOR NS0 CELL LINES EXPRESSING FULLY HUMAN ANTIBODIES AND GLUTAMINE SYNTHETASE (GS)
Jonathan Dempsey, Steve Ruddock, Matthew Osborne, Alison Ridley, Simone Sturt, Ray Field, Cambridge Antibody Technology, USA

II - 42. INTEGRATING ACOUSTIC PERFUSION IN MAMMALIAN CELL CULTURE. SCALE UP AND PERFORMANCE CHARACTERIZATION
T.M.P. Keijzer, A. Oudshoorn, H. van de Berg, AppliSens, THE NETHERLANDS
F. Trampler, Sonosep Technologies Inc., THE NETHERLANDS
O. Doblhoff-Dier, Institute for Applied Microbiology, THE NETHERLANDS

II - 43. COMPARISON OF BATCH AND PERFUSION CULTURE IN COMBINATION WITH PILOT SCALE EXPANDED BED PURIFICATION FOR THE PRODUCTION OF SOLUBLE RECOMBINANT B-SECRETASE
Elke Lüllau, Ari Kanttinen, Anna-Haag-Alvarsson, Jenny Hassel, Fritz Schweikart, AstraZeneca, SWEDEN
II - 44. STUDYING THE INSECT CELL METABOLISM USING AN ACCELERATION-STAT
Rolf Marteijn, Mariska Oude-Elferink, Dirk Martens, Hans Tramper, Wageningen University, THE NETHERLANDS

II - 45. SELECT- AN AUTOMATED CELL CULTURE SYSTEM FOR DRUG DISCOVERY
Julie Kerby, Emma Watts, Merck Sharp and Dohme Research Laboratories, UNITED KINGDOM

II - 46. CONTROLLED GROWTH OF ANIMAL CELLS FOR BIOPHARMACEUTICAL PRODUCTION
Ana Carvalhal, Jose Calado, Sonia Sa Santos, Manuel J.T. Carrondo, IBET/ITQB, PORTUGAL

II - 47. OPTIMIZATION OF LARGE-SCALE ULTRASONIC FILTER PERFORMANCE FOR A 100 L CHO CELL PERFUSION BIOREACTOR
Volker M. Gorenflo, Bruce D. Bowen, Sumitra Angepat, James M. Piret, University of British Columbia, CANADA

II - 48. OPTIMIZATION OF AN ACOUSTIC CELL FILTER WITH A NOVEL AIR-BACKFLUSH SYSTEM Volker M. Gorenflo, Bruce D. Bowen, Sumitra Angepat, James M. Piret, University of British Columbia, CANADA

II - 49. A RAPID AND RELIABLE NEW SEED-TRAIN EXPANSION METHOD FOR VARIOUS RECOMBINANT MAMMALIAN CELL LINES
Rüdiger Heidemann, Chun Zhang, James Michaels, Konstantin Konstantinov, Bayer Corp. Biotechnology Unit, USA
Hans-Jurgen Henzler, Bayer AG, GERMANY

II - 50. MONITORING PILOT SCALE CULTIVATIONS OF MAMMALIAN CELLS WITH AN AUTONOMOUS INDUSTRIAL ROBOT ARM
Iris Poggendorf, Dirk Luetkemeyer, Torsten Scherer, Alois Knoll, Juergen Lehmann, University of Bielefeld, GERMANY

II - 51. EVALUATION OF CELL SEPARATION DEVICES FOR THE PERFUSION OF ANIMAL CELL CULTURE IN BIOPHARMACEUTICAL PROCESSES
Veronique Chotteau, Torsten Bjorling, Andreas Gretander, Ola Tuvesson, Ulrich Dudel, Biovitrum, SWEDEN

II - 52. ON-LINE MEASUREMENT OF VIABLE CELL DENSITY IN MICRO-CARRIER AND PACKED BED CULTURE SYSTEMS
John Carvell, Aber Instruments, UNITED KINGDOM
Claire Lucas, Applikon Inc., USA
Richard Kemp, University of Aberystwyth, UNITED KINGDOM

II - 53. SHAKEN AND NOT STIRRED: EVALUATION OF VARIOUS SMALL AND MODERATE SCALE CULTIVATION SYSTEMS FOR SUSPENSION CELLS
Natalie Muller, Philippe Girard, Martin Jordan, Florian M. Wurm, EPFL, SWITZERLAND

II - 54. COMMERCIAL SCALE CELL CULTURE PRODUCTION MONITORING AND ANALYSIS
Tom Monica, Stacey Kaneshiro, Martin Gawlitzek, Raquel Iverson, Nitu Syal, Kirin Malik, Selene Perez, Cynthia Hoy, Arlis Corbett, Mike Estacio, Bob Kiss, Genentech, Inc., USA

II - 55. THE USE OF FLOW CYTOMETRIC ASSAYS TO MONITOR AND OPTIMIZE THAW CONDITIONS FOR MAMMALIAN CELL BANKS
Cynthia Hoy, Karen Kilplinger, Marcia Coyne, Robert Kiss, Genentech, Inc., USA

II - 56. SECRETED PRODUCTION OF HUMAN INTERLEUKIN-2 FUSED WITH GREEN FLUORESCENT PROTEIN IN STABLE INSECT DROSOPHILA S2 CELLS
Hyung Joon Cha, Hwa Sung Shin, Hye Jung Lim, Pohang University of Science and Technology, KOREA

II - 57. FROM BANK TO BULK IN A COMPLETELY DISPOSABLE PROCESS
Geoff Hodge, Frank Deer, John Kepler, Greg Papastoitsis, Millennium Pharmaceuticals, Inc., USA

II - 58. TROUBLE SHOOTING & SCALE-UP OF ANTIFOAM EFFECTIVENESS FOR CELL CULTURE PROCESSES
Thomas Black, Susan G. Brown, David T. Moore, Divakar, Ramakrishnan, Eli Lilly and Company, USA

II - 59. HEAT INACTIVATING MAMMALIAN CELL CULTURES FOR BIOWASTE KILL SYSTEM DESIGN
Niki Gregoriades, Melissa Luzardo, Beatrice Lucquet, Thomas Ryll, Abgenix Inc., USA

II - 60. ALTERNATING TANGENTIAL FLOW PERFUSION SYSTEM
Jean-Francois P. Hamel, Antonio Contreras, Jose M. Otero, MIT, USA

II - 61. FED-BATCH PROCESS FOR ENHANCING PRODUCTIVITY IN MAMMALIAN CELL CULTURE VIA ONLINE GLUTAMINE CONTROL
Yih Yean Lee, Danny C.F. Wong, Chun Loong Wong, Miranda G.S. Yap, Kathy T.K. Wong, National University of Singapore, SINGAPORE

II - 62. EVALUATION OF CELL COUNTING DEVICES FOR MAMMALIAN CELL CULTURE
Suzanne Kuo, Tom Stapp, Ron Taticek, Genentech, Inc., USA

II - 63. HIGH THROUGHPUT BIOPROCESSING USING NON-INVASIVE SENSORS
Govind Rao, Peter Harms, Yordan Kostov, Haley Kermis, Leah Tolosa, Atul Gupta, UMBC, USA

II - 64. HIGH THROUGHPUT CLONAL SELECTION OF RECOMBINANT CHO CELLS USING A DOMINANT SELECTABLE AND AMPLIFIABLE METALLOTHIONEIN-GFP FUSION PROTEIN
Noelle-Ann Sunstrom, Charles Bailey, Peter Gray, University of New South Wales, AUSTRALIA

II - 65. USE OF TWO DIMENSIONAL FACS FOR THE RAPID SELECTION OF CHO CLONES EXPRESSING RDNA ANTIBODIES AT HIGH LEVELS.
Peter P. Gray, Matthias Brand, Robert Gay, Noelle-Ann Sunstrom, University of New South Wales, AUSTRALIA

II - 66. FLUID MECHANICS, CELL DISTRIBUTION, AND ENVIRONMENT IN CELLCUBE BIOREACTORS
John Aunins, Maayan Katz, Merck and Company, Inc., USA
Brett Bader, Anthony Caola, Janet Griffiths, MIT, USA

II - 67. CHARACTERIZATION OF ANIMAL CELL BIOREACTORS WITH RESPECT TO LOCAL ENERGY DISSIPATION AND A PROPOSED, RATIONAL SCALE-UP/SCALE-DOWN METHODOLOGY
Jeffrey J Chalmers, Ohio State University, USA

II - 68. STRATEGIES FOR BETTER CO2 REMOVAL IN LARGE SCALE CULTURES
Sigma S. Mostafa, Jerry Yantis, Xuejun Gu, Eli Lilly & Company, USA
II - 69. QUANTITATIVE STUDIES OF MAMMALIAN CELL ATTACHMENT TO BUBBLE AND DAMAGE FROM GAS SPARGING
Ningning Ma, John G. Aunins, Weichang Zhou, Liangzhi Xie, Merck Research Laboratories, USA
Jeffrey J. Chalmers, Ohio State University, USA

II - 70. HEAT TRANSFER IN LARGE SCALE NUNC CELL FACTORY PRODUCTION PROCESSES
Colette S. Ranucci, William Adams, Sara Diffenbach, Ilse Blumentals, Shilpa Choudhari, Samantha Ozuna, Rosario Scott, Joan Whipple, Charles Goochee, Merck & Co., USA

II - 71. ULTRASONIC CELL SEPARATION FOR CONTINUOUS PERFUSION CULTURES
A.R. Bernard, H. Heine, C. Arod, Serono, SWITZERLAND
H.D. Blasey, CMS Siegfried, SWITZERLAND

II - 72. VERO CELL DETACHMENT IN LARGE SCALE NUNC CELL FACTORIES
Ilse I. Blumentals, C. Ranucci, S. Choudhari, A. Harris, E. Houghton, B. Mowrer, S. Ozuna, R. Scott, J. Whipple, G. Zang, J. Aunins, C. Goochee, Merck & Co., Inc., USA

II - 73. DEVELOPMENT OF NANOPARTICLE BASED SENSORS FOR TSES
Theresa Good, James Henry, Brad Atkinson, Rosana Moreira, Texas A & M University, USA

[ Poster Session I | Poster Session II | Final Program ]